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PMID: 18997027 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Two dissimilar N-acyl-homoserine lactone acylases of Pseudomonas syringae influence colony and biofilm morphology.

Applied and environmental microbiology ·Vol. 75 ·No. 1 ·2009-01-00 ·Pages 45-53

Shepherd RW, Lindow SE

Abstract

Plant aerial surfaces comprise a complex habitat for microorganisms, and many plant-associated bacteria, such as the pathogen Pseudomonas syringae, exhibit density-dependent survival on leaves by utilizing quorum sensing (QS). QS is often mediated by diffusible signals called N-acyl-homoserine lactones (AHLs), and P. syringae utilizes N-3-oxo-hexanoyl-dl-homoserine lactone (3OC6HSL) to control traits influencing epiphytic fitness and virulence. The P. syringae pathovar syringae B728a genome sequence revealed two putative AHL acylases, termed HacA (Psyr_1971) and HacB (Psyr_4858), which are N-terminal nucleophile hydrolases that inactivate AHLs by cleaving their amide bonds. HacA is a secreted AHL acylase that degrades only long-chain (C > or = 8) AHLs, while HacB is not secreted and degrades all tested AHLs. Targeted disruptions of hacA, hacB, and hacA and hacB together do not alter endogenous 3OC6HSL levels under the tested conditions. Surprisingly, targeted disruptions of hacA alone and hacA and hacB together confer complementable phenotypes that are very similar to autoaggregative phenotypes seen in other species. While AHL acylases might enable P. syringae B728a to degrade signals of competing species and block expression of their QS-dependent traits, these enzymes also play fundamental roles in biofilm formation.

MeSH Terms
4-Butyrolactone/analogs & derivatives,analysis Amidohydrolases/genetics,metabolism Biofilms/growth & development Cytoplasm/chemistry Gene Deletion Genetic Complementation Test Pseudomonas syringae/enzymology,genetics,growth & development Substrate Specificity
Chemicals
N-3-oxyhexanoyl-DL-homoserine lactone Amidohydrolases N-terminal nucleophile hydrolase 4-Butyrolactone
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shepherd Ryan W
Department of Plant and Microbial Biology, University of California-Berkeley, Berkeley, CA 94720-3102, USA.
Lindow Steven E
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
1098-5336
DOI
10.1128/AEM.01723-08
Published
2009-01-00
Epub
2008-00-07
Pages
45-53
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC2612208
Subset
IM
External Links
PubMed source DOI: 10.1128/AEM.01723-08 PMC Full Text View journal details
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