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PMID: 1898918 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Fnr mutants that activate gene expression in the presence of oxygen.

Journal of bacteriology ·Vol. 173 ·No. 1 ·1991-01-00 ·Pages 16-22

Kiley PJ, Reznikoff WS

Abstract

The regulatory protein Fnr is required for anaerobic expression of several anaerobic respiratory enzymes in Escherichia coli. To gain insight into how Fnr activity is regulated by oxygen, we have isolated Fnr mutants that increase expression of the nitrate reductase operon in the presence of oxygen (Fnr* mutants). Seven single-amino-acid substitutions that mapped within two regions of Fnr have been characterized. Two mutants mapped adjacent to two Cys residues in the N-terminal Cys cluster. Five Fnr* substitutions mapped to a region of Fnr that is similar to the cyclic AMP-binding domain of the catabolite activator protein (CAP). Within this group, four mutants were clustered in a region analogous to the CAP C helix, which is important in CAP dimer subunit interactions. Taken together, these data implicate regions in Fnr that may be important either in sensing oxygen deprivation or in the conformational change proposed to be necessary for Fnr activation under anaerobic conditions.

Related Genes
fnr
MeSH Terms
Aerobiosis Allosteric Site Amino Acid Sequence Anaerobiosis Bacterial Proteins/genetics,metabolism Base Sequence DNA, Bacterial/genetics,isolation & purification Escherichia coli/genetics,metabolism Escherichia coli Proteins Gene Expression Regulation, Bacterial Genes, Bacterial Iron-Sulfur Proteins Molecular Sequence Data Mutagenesis Nitrate Reductases/genetics Operon Plasmids Restriction Mapping Transcription Factors/genetics beta-Galactosidase/genetics,metabolism
Chemicals
Bacterial Proteins DNA, Bacterial Escherichia coli Proteins FNR protein, E coli Iron-Sulfur Proteins Transcription Factors Nitrate Reductases beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kiley P J
Department of Biochemistry, University of Wisconsin, Madison 53706.
Reznikoff W S
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.1.16-22.1991
Published
1991-01-00
Pages
16-22
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207150
Subset
IM
Grants
NIGMS NIH HHS · GM12953 · United States
NIGMS NIH HHS · GM19670 · United States
External Links
PubMed source DOI: 10.1128/jb.173.1.16-22.1991 PMC Full Text View journal details
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