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PMID: 1895401 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Inhibition of human immunodeficiency virus type 1 multiplication by antisense and sense RNA expression.

Journal of virology ·Vol. 65 ·No. 10 ·1991-10-00 ·Pages 5524-30

Joshi S, Van Brunschot A, Asad S, van der Elst I, Read SE, Bernstein A

Abstract

Human immunodeficiency virus type 1 (HIV-1) primarily infects CD4+ lymphocytes and macrophages and causes AIDS in humans. Retroviral vectors allowing neomycin phosphotransferase (npt) gene expression were engineered to express 5' sequences of HIV-1 RNA in the antisense or sense orientation and used to transform the human CD4+ lymphocyte-derived MT4 cell line. Cells expressing antisense or sense RNA to the HIV-1 tat mRNA leader sequence, as part of the 3' untranslated region of the npt mRNA, remained sensitive to HIV-1 infection. In contrast, resistance to HIV-1 infection was observed in cells expressing antisense RNA to the HIV-1 primer-binding site or to the region 5' to the primer-binding site as part of the 3' region of the npt mRNA. Cells expressing the tat mRNA leader sequence in the sense orientation as a precise replacement of the 5' untranslated region of npt mRNA were also resistant to HIV-1. These results indicate that sense and antisense approaches can be used to interfere with HIV-1 multiplication.

MeSH Terms
Base Sequence Blotting, Northern Cell Line Cell Transformation, Viral Gene Expression Genes, Viral Genetic Vectors HIV-1/genetics,physiology Humans Kinetics Molecular Sequence Data Oligonucleotide Probes Plasmids RNA, Antisense/genetics RNA, Viral/genetics Restriction Mapping Virus Replication/genetics
Chemicals
Oligonucleotide Probes RNA, Antisense RNA, Viral
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Joshi S
Department of Microbiology, University of Toronto, Ontario, Canada.
Van Brunschot A
Asad S
van der Elst I
Read S E
Bernstein A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.10.5524-5530.1991
Published
1991-10-00
Pages
5524-30
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249050
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.65.10.5524-5530.1991 PMC Full Text View journal details
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