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PMID: 1893793 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

CENP-B is a highly conserved mammalian centromere protein with homology to the helix-loop-helix family of proteins.

Chromosoma ·Vol. 100 ·No. 6 ·1991-07-00 ·Pages 360-70

Sullivan KF, Glass CA

Abstract

CENP-B is a centromere associated protein originally identified in human cells as an 80 kDa autoantigen recognized by sera from patients with anti-centromere antibodies (ACA). Recent evidence indicates that CENP-B interacts with centromeric heterochromatin in human chromosomes and may bind to a specific subset of human alphoid satellite DNA. CENP-B has not been unambiguously identified in non-primates and could, in principal, be a primate-specific alphoid DNA binding protein. In this work, a human genomic DNA segment containing the CENP-B gene was isolated and subjected to DNA sequence analysis. In vitro expression identified the site for translation initiation of CENP-B, demonstrating that it is encoded by an intronless open reading frame (ORF) in human DNA. A homologous mouse gene was also isolated and characterized. It was found to possess a high degree of homology with the human gene, containing an intronless ORF coding for a 599 residue polypeptide with 96% sequence similarity to human CENP-B. 5' and 3' flanking and untranslated sequences were conserved at a level of 94.6% and 82.7%, respectively, suggesting that the regulatory properties of CENP-B may be conserved as well. CENP-B mRNA was detected in mouse cells and tissues and an immunoreactive nuclear protein identical in size to human CENP-B was detected in mouse 3T3 cells using human ACA. Analysis of the sequence of CENP-B revealed a segment of significant similarity to a DNA binding motif identified for the helix-loop-helix (HLH) family of DNA binding proteins. These data demonstrate that CENP-B is a highly conserved mammalian protein that may be a member of the HLH protein family and suggest that it plays a role in a conserved aspect of centromere structure or function.

MeSH Terms
Amino Acid Sequence Animals Autoantigens/genetics,isolation & purification Base Sequence Centromere/chemistry Centromere Protein B Chromosomal Proteins, Non-Histone/genetics,isolation & purification Cloning, Molecular DNA-Binding Proteins Genes HeLa Cells Humans Lymphocytes/chemistry Mice Molecular Sequence Data Protein Conformation Sequence Homology, Nucleic Acid Species Specificity Structure-Activity Relationship
Chemicals
Autoantigens CENPB protein, human Cenpb protein, mouse Centromere Protein B Chromosomal Proteins, Non-Histone DNA-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sullivan K F
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Glass C A
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00337514
Published
1991-07-00
Pages
360-70
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
Grants
NIGMS NIH HHS · GM36098 · United States
Databases
GENBANK
S55742, S55745, S60911, S60914, S60917, S60919, S60920, S60969, X55038, X55039
External Links
PubMed source DOI: 10.1007/BF00337514 View journal details
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