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PMID: 18926722 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Metabolite imaging using matrix-enhanced surface-assisted laser desorption/ionization mass spectrometry (ME-SALDI-MS).

Journal of the American Society for Mass Spectrometry ·Vol. 20 ·No. 1 ·2009-01-00 ·Pages 80-8

Liu Q, Xiao Y, Pagan-Miranda C, Chiu YM, He L

Abstract

We describe here the use of a hybrid ionization approach, matrix-enhanced surface-assisted laser desorption/ionization mass spectrometry (ME-SALDI-MS) in bioimaging. ME-SALDI combines the strengths of traditional matrix-assisted laser desorption/ionization (MALDI) and SALDI and enables successful MS imaging of low-mass species with improved detection sensitivity. Using 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) as the MS standard, MS performances of MALDI, SALDI, and ME-SALDI are systematically compared. The analyte desorption and ionization mechanism in ME-SALDI is qualitatively speculated based on the observation of significantly reduced matrix background and improved survival yields of molecular ions. Improvements in detection sensitivity of low-mass species using ME-SALDI over MALDI in imaging are demonstrated with mouse heart and brain tissues.

MeSH Terms
Animals Brain Chemistry Cerebellum/chemistry Cerebrum/chemistry Diagnostic Imaging/methods Dimyristoylphosphatidylcholine/chemistry Female Mice Myocardium/chemistry Sensitivity and Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
Chemicals
Dimyristoylphosphatidylcholine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Liu Qiang
Department of Chemistry, North Carolina State University, Raleigh, North Carolina, USA.
Xiao Yongsheng
Pagan-Miranda Coral
Chiu Yu Matthew
He Lin
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Article Info
Journal
Journal of the American Society for Mass Spectrometry
Abbr.
J Am Soc Mass Spectrom
ISSN
1044-0305
DOI
10.1016/j.jasms.2008.09.011
Published
2009-01-00
Epub
2008-00-20
Pages
80-8
Language
English
Region
United States
NLM ID
9010412
Subset
IM
External Links
PubMed source DOI: 10.1016/j.jasms.2008.09.011 View journal details
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