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PMID: 15232100 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Mass spectrometric imaging of highly curved membranes during Tetrahymena mating.

Science (New York, N.Y.) ·Vol. 305 ·No. 5680 ·2004-07-02 ·Pages 71-3

Ostrowski SG, Van Bell CT, Winograd N, Ewing AG

Abstract

Biological membrane fusion is crucial to numerous cellular events, including sexual reproduction and exocytosis. Here, mass spectrometry images demonstrate that the low-curvature lipid phosphatidylcholine is diminished in the membrane regions between fusing Tetrahymena, where a multitude of highly curved fusion pores exist. Additionally, mass spectra and principal component analysis indicate that the fusion region contains elevated amounts of 2-aminoethylphosphonolipid, a high-curvature lipid. This evidence suggests that biological fusion involves and might in fact be driven by a heterogeneous redistribution of lipids at the fusion site.

MeSH Terms
Animals Cell Membrane/chemistry,ultrastructure Freeze Fracturing Lipid Bilayers Membrane Fusion Membrane Lipids/analysis Phosphatidylcholines/analysis Phospholipids/analysis Principal Component Analysis Spectrometry, Mass, Secondary Ion Tetrahymena thermophila/chemistry,physiology,ultrastructure
Chemicals
2-aminoethylphosphonolipid Lipid Bilayers Membrane Lipids Phosphatidylcholines Phospholipids
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ostrowski Sara G
Department of Chemistry, Pennsylvania State University, University Park, PA 16802, USA.
Van Bell Craig T
Winograd Nicholas
Ewing Andrew G
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Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
DOI
10.1126/science.1099791
Published
2004-07-02
Pages
71-3
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC2833272
Subset
IM
Grants
NIBIB NIH HHS · R01 EB002016 · United States
NIBIB NIH HHS · R01 EB002016-11 · United States
External Links
PubMed source DOI: 10.1126/science.1099791 PMC Full Text View journal details
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