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PMID: 18852876 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Epithelial to mesenchymal transition of a primary prostate cell line with switches of cell adhesion modules but without malignant transformation.

PloS one ·Vol. 3 ·No. 10 ·2008-00-00 ·Pages e3368

Ke XS, Qu Y, Goldfinger N, Rostad K, Hovland R, Akslen LA, Rotter V, Øyan AM, Kalland KH

Abstract

Epithelial to mesenchymal transition (EMT) has been connected with cancer progression in vivo and the generation of more aggressive cancer cell lines in vitro. EMT has been induced in prostate cancer cell lines, but has previously not been shown in primary prostate cells. The role of EMT in malignant transformation has not been clarified. In a transformation experiment when selecting for cells with loss of contact inhibition, the immortalized prostate primary epithelial cell line, EP156T, was observed to undergo EMT accompanied by loss of contact inhibition after about 12 weeks in continuous culture. The changed new cells were named EPT1. EMT of EPT1 was characterized by striking morphological changes and increased invasion and migration compared with the original EP156T cells. Gene expression profiling showed extensively decreased epithelial markers and increased mesenchymal markers in EPT1 cells, as well as pronounced switches of gene expression modules involved in cell adhesion and attachment. Transformation assays showed that EPT1 cells were sensitive to serum or growth factor withdrawal. Most importantly, EPT1 cells were not able to grow in an anchorage-independent way in soft agar, which is considered a critical feature of malignant transformation. This work for the first time established an EMT model from primary prostate cells. The results show that EMT can be activated as a coordinated gene expression program in association with early steps of transformation. The model allows a clearer identification of the molecular mechanisms of EMT and its potential role in malignant transformation.

MeSH Terms
Cell Adhesion/genetics Cell Line Cell Movement Cell Shape Cell Transformation, Neoplastic/genetics Epithelial Cells/cytology Gene Expression Profiling Humans Male Mesenchymal Stem Cells/cytology Prostate/cytology,metabolism
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Ke Xi-Song
The Gade Institute, University of Bergen, Bergen, Norway.
Qu Yi
Goldfinger Naomi
Rostad Kari
Hovland Randi
Akslen Lars A
Rotter Varda
Øyan Anne Margrete
Kalland Karl-Henning
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0003368
Published
2008-00-00
Epub
2008-00-13
Pages
e3368
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC2557125
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pone.0003368 PMC Full Text View journal details
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