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PMID: 18799745 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The structure of a complete phytochrome sensory module in the Pr ground state.

Essen LO, Mailliet J, Hughes J

Abstract

Phytochromes are red/far-red photochromic biliprotein photoreceptors, which in plants regulate seed germination, stem extension, flowering time, and many other light effects. However, the structure/functional basis of the phytochrome photoswitch is still unclear. Here, we report the ground state structure of the complete sensory module of Cph1 phytochrome from the cyanobacterium Synechocystis 6803. Although the phycocyanobilin (PCB) chromophore is attached to Cys-259 as expected, paralleling the situation in plant phytochromes but contrasting to that in bacteriophytochromes, the ZZZssa conformation does not correspond to that expected from Raman spectroscopy. We show that the PHY domain, previously considered unique to phytochromes, is structurally a member of the GAF (cGMP phosphodiesterase/adenylyl cyclase/FhlA) family. Indeed, the tandem-GAF dumbbell revealed for phytochrome sensory modules is remarkably similar to the regulatory domains of cyclic nucleotide (cNMP) phosphodiesterases and adenylyl cyclases. A unique feature of the phytochrome structure is a long, tongue-like protrusion from the PHY domain that seals the chromophore pocket and stabilizes the photoactivated far-red-absorbing state (Pfr). The tongue carries a conserved PRxSF motif, from which an arginine finger points into the chromophore pocket close to ring D forming a salt bridge with a conserved aspartate residue. The structure that we present provides a framework for light-driven signal transmission in phytochromes.

MeSH Terms
Bacterial Proteins/chemistry Models, Molecular Photoreceptors, Microbial Phytochrome/chemistry Protein Kinases/chemistry Protein Structure, Tertiary Recombinant Proteins/chemistry Signal Transduction Synechocystis/chemistry
Chemicals
Bacterial Proteins Photoreceptors, Microbial Recombinant Proteins Phytochrome Protein Kinases Cph1 phytochrome protein, bacteria
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Essen Lars-Oliver
Structural Biochemistry, Department of Chemistry, Philipps University, Hans-Meerwein-Strasse, D-35032 Marburg, Germany. essen@chemie.uni-marburg.de
Mailliet Jo
Hughes Jon
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
Published
2008-09-23
Epub
2008-00-17
Pages
14709-14
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2567182
Subset
IM
Databases
PDB
Analysis Services
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