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PMID: 18692470 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Structural basis for cargo regulation of COPII coat assembly.

Cell ·Vol. 134 ·No. 3 ·2008-08-08 ·Pages 474-84

Stagg SM, LaPointe P, Razvi A, Gürkan C, Potter CS, Carragher B, Balch WE

Abstract

Using cryo-electron microscopy, we have solved the structure of an icosidodecahedral COPII coat involved in cargo export from the endoplasmic reticulum (ER) coassembled from purified cargo adaptor Sec23-24 and Sec13-31 lattice-forming complexes. The coat structure shows a tetrameric assembly of the Sec23-24 adaptor layer that is well positioned beneath the vertices and edges of the Sec13-31 lattice. Fitting the known crystal structures of the COPII proteins into the density map reveals a flexible hinge region stemming from interactions between WD40 beta-propeller domains present in Sec13 and Sec31 at the vertices. The structure shows that the hinge region can direct geometric cage expansion to accommodate a wide range of bulky cargo, including procollagen and chylomicrons, that is sensitive to adaptor function in inherited disease. The COPII coat structure leads us to propose a mechanism by which cargo drives cage assembly and membrane curvature for budding from the ER.

MeSH Terms
Carrier Proteins/chemistry,metabolism Cryoelectron Microscopy Endoplasmic Reticulum/chemistry,metabolism Humans Models, Molecular Protein Transport Vesicular Transport Proteins/chemistry,metabolism
Chemicals
Carrier Proteins SEC13 protein, human SEC23A protein, human SEC24C protein, human SEC31A protein, human Vesicular Transport Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Stagg Scott M
National Resource for Automated Molecular Microscopy, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
LaPointe Paul
Razvi Abbas
Gürkan Cemal
Potter Clinton S
Carragher Bridget
Balch William E
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
1097-4172
DOI
10.1016/j.cell.2008.06.024
Published
2008-08-08
Pages
474-84
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC2649882
Subset
IM
Grants
NIGMS NIH HHS · R01 GM042336 · United States
NCRR NIH HHS · P41 RR017573 · United States
NIGMS NIH HHS · GM42336 · United States
NIGMS NIH HHS · F32 GM073509 · United States
NCRR NIH HHS · P41 RR017573-07 · United States
NIBIB NIH HHS · R33 EB000798-04 · United States
NIBIB NIH HHS · R33 EB000798 · United States
NIGMS NIH HHS · R01 GM042336-17S1 · United States
NIGMS NIH HHS · GM073509 · United States
NCRR NIH HHS · RR17573 · United States
NIGMS NIH HHS · F32 GM073509-02 · United States
NIBIB NIH HHS · R33EB00798 · United States
Corrections
CommentIn
PMID: 18692460
External Links
PubMed source DOI: 10.1016/j.cell.2008.06.024 PMC Full Text View journal details
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