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PMID: 1868840 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The replication termination signal terB of the Escherichia coli chromosome is a deletion hot spot.

The EMBO journal ·Vol. 10 ·No. 9 ·1991-09-00 ·Pages 2699-705

Bierne H, Ehrlich SD, Michel B

Abstract

Hybrids composed of phage M13, plasmid pBR322 and the termination signal of Escherichia coli chromosome replication terB were used to show that arrest of DNA synthesis creates a very efficient deletion hot spot. Up to 80% of deletions occurring in these hybrids had one deletion end-point at terB provided that (i) terB was oriented to arrest M13 and pBR322 leading strand synthesis; and (ii) the host cells contained the Tus protein necessary for arresting DNA synthesis at terB. The position of terB and the flanking sequences had little effect on deletion hot spot activity. About 90% of the deletions at terB ended 5-6 nucleotides in front of the major replication arrest site. We propose two models to account for deletion formation and speculate that many genome rearrangements may be due to the pausing of DNA replication.

Related Genes
terB
MeSH Terms
Base Sequence Chromosome Deletion Chromosome Mapping Chromosomes, Bacterial DNA Replication DNA, Bacterial/biosynthesis Escherichia coli/genetics Molecular Sequence Data Plasmids Recombination, Genetic
Chemicals
DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bierne H
Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.
Ehrlich S D
Michel B
References (29)
29 references, click to expand
  1. Genetic recombination of bacterial plasmid DNA. Analysis of the effect of recombination-deficient mutations on plasmid recombination.
    J Mol Biol. 1982 Sep 25;160(3):411-30 PMID: 6759658
  2. A conserved sequence at c-myc oncogene chromosomal translocation breakpoints in plasmacytomas.
    Nature. 1984 Jul 26-Aug 1;310(5975):327-30 PMID: 6462219
  3. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  4. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.
    Nucleic Acids Res. 1978 Aug;5(8):2721-8 PMID: 358147
  5. Genetic recombination of bacterial plasmid DNA: effect of RecF pathway mutations on plasmid recombination in Escherichia coli.
    J Bacteriol. 1985 Sep;163(3):1060-6 PMID: 2993230
  6. Deletion hot spots in chimeric Escherichia coli plasmids.
    J Bacteriol. 1989 Apr;171(4):1846-53 PMID: 2649476
  7. Role of chromosome translocations in human neoplasia.
    Cell. 1987 Apr 24;49(2):155-6 PMID: 3494520
  8. Identification of the DNA sequence from the E. coli terminus region that halts replication forks.
    Cell. 1988 Nov 4;55(3):459-66 PMID: 2846183
  9. Illegitimate recombination at the replication origin of bacteriophage M13.
    Proc Natl Acad Sci U S A. 1986 May;83(10):3386-90 PMID: 3010295
  10. Escherichia coli topoisomerase I can segregate replicating pBR322 daughter DNA molecules in vitro.
    J Biol Chem. 1986 Sep 5;261(25):11906-17 PMID: 3017951
  11. Intramolecular recombination of linear DNA catalyzed by the Escherichia coli RecE recombination system.
    J Mol Biol. 1985 Dec 5;186(3):515-25 PMID: 3005590
  12. Bacteriophage T4 DNA topoisomerase mediates illegitimate recombination in vitro.
    Proc Natl Acad Sci U S A. 1986 Feb;83(4):922-6 PMID: 3006033
  13. Association of crossover points with topoisomerase I cleavage sites: a model for nonhomologous recombination.
    Science. 1985 Nov 22;230(4728):954-8 PMID: 2997924
  14. Sequence features of the replication terminus of the Bacillus subtilis chromosome.
    Nucleic Acids Res. 1987 Oct 26;15(20):8501-9 PMID: 3118336
  15. Core sequence of two separable terminus sites of the R6K plasmid that exhibit polar inhibition of replication is a 20 bp inverted repeat.
    Cell. 1988 Aug 12;54(4):515-23 PMID: 3042153
  16. Multiple mechanisms for initiation of ColE1 DNA replication: DNA synthesis in the presence and absence of ribonuclease H.
    Cell. 1987 Dec 24;51(6):1113-22 PMID: 2446774
  17. Escherichia coli replication termination protein impedes the action of helicases.
    Proc Natl Acad Sci U S A. 1989 Dec;86(23):9104-8 PMID: 2556700
  18. Termination of DNA replication in Escherichia coli requires a trans-acting factor.
    J Bacteriol. 1988 Feb;170(2):662-8 PMID: 3276664
  19. Escherichia coli Tus protein acts to arrest the progression of DNA replication forks in vitro.
    Proc Natl Acad Sci U S A. 1990 Apr;87(7):2481-5 PMID: 2181438
  20. Tetracycline resistance determined by pBR322 is mediated by one polypeptide.
    Gene. 1983 Dec;26(2-3):197-203 PMID: 6323260
  21. Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance.
    J Bacteriol. 1982 May;150(2):815-25 PMID: 6950931
  22. Site-specific pausing of deoxyribonucleic acid synthesis catalyzed by four forms of Escherichia coli DNA polymerase III.
    Biochemistry. 1983 Oct 25;22(22):5177-88 PMID: 6360204
  23. Replication functions of pC194 are necessary for efficient plasmid transduction by M13 phage.
    EMBO J. 1984 Jan;3(1):81-6 PMID: 6323171
  24. In vitro study of illegitimate recombination: involvement of DNA gyrase.
    Cold Spring Harb Symp Quant Biol. 1981;45 Pt 1:399-408 PMID: 6271485
  25. Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide, and streptogramin type B antibodies.
    J Bacteriol. 1982 May;150(2):804-14 PMID: 6279574
  26. Role for DNA homology in site-specific recombination. The isolation and characterization of a site affinity mutant of coliphage lambda.
    J Mol Biol. 1983 Oct 25;170(2):319-42 PMID: 6226804
  27. Nucleotide sequence of the filamentous bacteriophage M13 DNA genome: comparison with phage fd.
    Gene. 1980 Oct;11(1-2):129-48 PMID: 6254849
  28. The functional origin of bacteriophage f1 DNA replication. Its signals and domains.
    J Mol Biol. 1984 Feb 5;172(4):507-21 PMID: 6607361
  29. The nucleotide sequence surrounding the replication terminus of R6K.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2095-9 PMID: 6941271
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-09-00
Pages
2699-705
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452973
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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