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PMID: 18669478 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Global organization of replication time zones of the mouse genome.

Genome research ·Vol. 18 ·No. 10 ·2008-10-00 ·Pages 1562-70

Farkash-Amar S, Lipson D, Polten A, Goren A, Helmstetter C, Yakhini Z, Simon I

Abstract

The division of genomes into distinct replication time zones has long been established. However, an in-depth understanding of their organization and their relationship to transcription is incomplete. Taking advantage of a novel synchronization method ("baby machine") and of genomic DNA microarrays, we have, for the first time, mapped replication times of the entire mouse genome at a high temporal resolution. Our data revealed that although most of the genome has a distinct time of replication either early, middle, or late S phase, a significant portion of the genome is replicated asynchronously. Analysis of the replication map revealed the genomic scale organization of the replication time zones. We found that the genomic regions between early and late replication time zones often consist of extremely large replicons. Analysis of the relationship between replication and transcription revealed that early replication is frequently correlated with the transcription potential of a gene and not necessarily with its actual transcriptional activity. These findings, along with the strong conservation found between replication timing in human and mouse genomes, emphasize the importance of replication timing in transcription regulation.

MeSH Terms
Animals Chromosome Mapping DNA Replication DNA Replication Timing Genome In Situ Hybridization, Fluorescence Mice Replication Origin Transcription, Genetic
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Farkash-Amar Shlomit
Department of Molecular Biology, Hebrew University Medical School Jerusalem 91120, Israel.
Lipson Doron
Polten Andreas
Goren Alon
Helmstetter Charles
Yakhini Zohar
Simon Itamar
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Article Info
Journal
Genome research
Abbr.
Genome Res
ISSN
1088-9051
DOI
10.1101/gr.079566.108
Published
2008-10-00
Epub
2008-00-30
Pages
1562-70
Language
English
Region
United States
NLM ID
9518021
PMCID
PMC2556267
Subset
IM
External Links
PubMed source DOI: 10.1101/gr.079566.108 PMC Full Text View journal details
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