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PMID: 1864975 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Acute lung injury. Pathogenesis of intraalveolar fibrosis.

The Journal of clinical investigation ·Vol. 88 ·No. 2 ·1991-08-00 ·Pages 663-73

Snyder LS, Hertz MI, Peterson MS, Harmon KR, Marinelli WA, Henke CA, Greenheck JR, Chen B, Bitterman PB

Abstract

In patients dying with acute lung injury, interstitial mesenchymal cells migrate into the airspace where they replicate and deposit connective tissue. We therefore hypothesized that peptides capable of promoting mesenchymal cell migration and replication would be present in the alveolar airspace. To examine this hypothesis, patients with severe acute diffuse lung injury (n = 26) underwent bronchoalveolar lavage. Acutely ill patients without lung injury served as controls (n = 12). Recovered effluent was examined for mesenchymal cell growth-promoting and migration-promoting activity. Lavage cell supernates from both patients and controls were devoid of bioactivity. However, substantial growth-promoting and migration-promoting activity was present in lavage fluid from nearly every patient, whereas little or none was present in fluid from controls. Characterization of the bioactivity indicated a significant proportion consisted of three peptides related to PDGF: (a) a 14-kD peptide that shared with PDGF several biophysical, biochemical, receptor-binding, and antigenic properties; (b) a 29-kD peptide that appeared identical to PDGF of platelet origin; and (c) a 38-kD peptide that was biophysically and antigenically similar to PDGF. These data indicate that peptide moieties are present in the airspace of patients after acute lung injury that can signal mesenchymal cell migration and replication.

MeSH Terms
Adolescent Adult Aged Bronchoalveolar Lavage Fluid Cell Division Cell Movement Female Fibroblasts Granulation Tissue Humans Immunoblotting Male Middle Aged Platelet-Derived Growth Factor/metabolism Pulmonary Fibrosis/etiology,pathology Radioligand Assay
Chemicals
Platelet-Derived Growth Factor
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Snyder L S
Department of Medicine, University of Minnesota, Minneapolis 55455.
Hertz M I
Peterson M S
Harmon K R
Marinelli W A
Henke C A
Greenheck J R
Chen B
Bitterman P B
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI115351
Published
1991-08-00
Pages
663-73
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC295409
Subset
IM
Grants
NHLBI NIH HHS · 5F32 HL-07621 · United States
NHLBI NIH HHS · 5R01 HL-39833 · United States
NHLBI NIH HHS · NRSA F32 HL-08051 · United States
External Links
PubMed source DOI: 10.1172/JCI115351 PMC Full Text View journal details
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