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PMID: 2734294 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Two peptides related to platelet-derived growth factor are present in human wound fluid.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 12 ·1989-06-00 ·Pages 4416-20

Matsuoka J, Grotendorst GR

Abstract

Platelet-derived growth factor (PDGF) has been suggested to play an important role in wound healing. In this study wound fluid collected from patients after radical mastectomy was analyzed for PDGF-related factors. Analysis revealed the presence of two PDGF-related peptides of 16-17 kDa and 34-36 kDa. These PDGF-related peptides were purified by using anti-PDGF immunoaffinity techniques and found to possess both chemotactic and mitogenic activities for NIH 3T3 cells in vitro. Surprisingly, we could not detect authentic native PDGF (30 kDa) or PDGF A or B chain in any of the wound-fluid samples examined. These results indicate that PDGF-related peptides are present in normal human wound fluid, whereas PDGF A- and B-chain peptides are absent or only present in trace amounts relative to the total PDGF-related biological activity.

MeSH Terms
Animals Blotting, Western Cells, Cultured Chemotaxis/drug effects Chromatography, Affinity Female Humans Macromolecular Substances Mastectomy, Radical Mice Molecular Weight Platelet-Derived Growth Factor/analogs & derivatives,immunology,isolation & purification,pharmacology Wounds and Injuries/physiopathology
Chemicals
Macromolecular Substances Platelet-Derived Growth Factor
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Matsuoka J
Department of Biology, University of South Florida, Tampa 33620.
Grotendorst G R
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18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.12.4416
Published
1989-06-00
Pages
4416-20
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC287280
Subset
IM
Grants
NIGMS NIH HHS · GM 37223 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.12.4416 PMC Full Text View journal details
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