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PMID: 1864603 Published · ppublish English Case Reports Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of seven DA/DAPI-positive bisatellited marker chromosomes by in situ hybridization.

Human genetics ·Vol. 87 ·No. 3 ·1991-07-00 ·Pages 290-6

Plattner R, Heerema NA, Patil SR, Howard-Peebles PN, Palmer CG

Abstract

Seven dicentric bisatellited marker chromosomes, ascertained at amniocentesis, chorionic villus sampling, and in blood from an abnormal liveborn were characterized cytogenetically. All seven markers demonstrated brilliant bands by the DA/DAPI technique corresponding to C-band positive regions. Although some dicentric DA/DAPI-positive bisatellited markers have been identified as inverted duplicated 15s, recent literature has suggested that DA/DAPI lacks specificity for chromosome 15. Our evaluation of DA/DAPI-positive bisatellited marker chromosomes by in situ hybridization shows that some originate from chromosome 15 whereas DA/DAPI negative bisatellited markers may not be derived from 15. The morphological variations noted in our studies are discussed with respect to nomenclature.

MeSH Terms
Amniocentesis Child, Preschool Chorionic Villi Sampling Chromosome Banding Chromosomes, Human, Pair 15 DNA Probes DNA, Satellite/analysis Distamycins Female Fluorescent Dyes Genetic Markers Humans Indoles Karyotyping Nucleic Acid Hybridization Pregnancy
Chemicals
DNA Probes DNA, Satellite Distamycins Fluorescent Dyes Genetic Markers Indoles DAPI stallimycin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Plattner R
Department of Medical Genetics, Indiana School of Medicine, Indianapolis 46202.
Heerema N A
Patil S R
Howard-Peebles P N
Palmer C G
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Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00200906
Published
1991-07-00
Pages
290-6
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
Grants
NICHD NIH HHS · 1T32HD07373 · United States
External Links
PubMed source DOI: 10.1007/BF00200906 View journal details
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