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PMID: 1863767 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of novel genes specifically expressed in Chlamydomonas reinhardtii zygotes.

Plant molecular biology ·Vol. 16 ·No. 6 ·1991-06-00 ·Pages 937-46

Wegener D, Beck CF

Abstract

The maturation of zygotes formed by the fusion of two gametes is the essential part of the diploid phase of the Chlamydomonas reinhardtii sexual life cycle and results in mature zygotes competent to germinate. To understand the molecular mechanisms underlying zygote maturation and the attainment of competence for germination we isolated genomic clones representing three different genes that are specifically expressed in Chlamydomonas reinhardtii zygotes. Accumulation of the RNAs started more than 24 h after mating, setting these genes apart from genes expressed in young zygotes. Upon light-induced germination of zygotes, the mRNAs disappeared. The patterns of RNA accumulation and disappearance were gene-specific and suggested a function of these genes in maturation and/or in initial steps of germination.

MeSH Terms
Blotting, Northern Chlamydomonas/genetics,growth & development,physiology Cloning, Molecular Genes, Plant/genetics Transcription, Genetic/physiology
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wegener D
Institut für Biologie III, Albert-Ludwigs-Universität, Freiburg, Germany.
Beck C F
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00016066
Published
1991-06-00
Pages
937-46
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00016066 View journal details
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