Home LiteratureArticle Details
PMID: 18628919 Published · ppublish English Journal Article

Comparison of the proteomes of three yeast wild type strains: CEN.PK2, FY1679 and W303.

Comparative and functional genomics ·Vol. 2 ·No. 4 ·2001-00-00 ·Pages 207-25

Rogowska-Wrzesinska A, Larsen PM, Blomberg A, Görg A, Roepstorff P, Norbeck J, Fey SJ

Abstract

Yeast deletion strains created during gene function analysis projects very often show drastic phenotypic differences depending on the genetic background used. These results indicate the existence of important molecular differences between the CEN.PK2, FY1679 and W303 wild type strains. To characterise these differences we have compared the protein expression levels between CEN.PK2, FY1679 and W303 strains using twodimensional gel electrophoresis and identified selected proteins by mass spectrometric analysis. We have found that FY1679 and W303 strains are more similar to each other than to the CEN.PK2 strain. This study identifies 62 proteins that are differentially expressed between the strains and provides a valuable source of data for the interpretation of yeast mutant phenotypes observed in CEN.PK2, FY1679 and W303 strains.

Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Rogowska-Wrzesinska A
Centre for Proteome Analysis in Life Sciences, University of Southern Denmark, International Science Park Odense, Forskerparken 10B, Odense M 5230, Denmark. ar@cpa.spo.dk
Larsen P M
Blomberg A
Görg A
Roepstorff P
Norbeck J
Fey S J
References (46)
46 references, click to expand
  1. Identification and specificities of N-terminal acetyltransferases from Saccharomyces cerevisiae.
    EMBO J. 1999 Nov 1;18(21):6155-68 PMID: 10545125
  2. YPD, PombePD and WormPD: model organism volumes of the BioKnowledge library, an integrated resource for protein information.
    Nucleic Acids Res. 2001 Jan 1;29(1):75-9 PMID: 11125054
  3. Two actin-related proteins are shared functional components of the chromatin-remodeling complexes RSC and SWI/SNF.
    Mol Cell. 1998 Nov;2(5):639-51 PMID: 9844636
  4. Disruption and phenotypic analysis of seven ORFs from the left arm of chromosome XV of Saccharomyces cerevisiae.
    Yeast. 1999 Mar 30;15(5):435-41 PMID: 10220002
  5. The effect of ochre suppression on meiosis and ascospore formation in Saccharomyces.
    Genetics. 1977 Jan;85(1):35-54 PMID: 320093
  6. Specificity of cotranslational amino-terminal processing of proteins in yeast.
    Biochemistry. 1987 Dec 15;26(25):8242-6 PMID: 3327521
  7. Yeast MAK3 N-acetyltransferase recognizes the N-terminal four amino acids of the major coat protein (gag) of the L-A double-stranded RNA virus.
    J Bacteriol. 1993 May;175(10):3192-4 PMID: 8491733
  8. Two-dimensional gel protein database of Saccharomyces cerevisiae (update 1999).
    Electrophoresis. 1999 Aug;20(11):2280-98 PMID: 10493132
  9. Dependence on mating type for the overproduction of iso-2-cytochrome c in the yeast mutant CYC7-H2.
    Genetics. 1980 Apr;94(4):891-8 PMID: 6254832
  10. Genealogy of principal strains of the yeast genetic stock center.
    Genetics. 1986 May;113(1):35-43 PMID: 3519363
  11. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  12. Multiple signals regulate GAL transcription in yeast.
    Mol Cell Biol. 2000 Jun;20(11):3880-6 PMID: 10805731
  13. A 'natural' mutation in Saccharomyces cerevisiae strains derived from S288c affects the complex regulatory gene HAP1 (CYP1).
    Curr Genet. 1999 Oct;36(4):195-200 PMID: 10541856
  14. Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C.
    Yeast. 1995 Jan;11(1):53-5 PMID: 7762301
  15. Protection from nitrosative stress by yeast flavohemoglobin.
    Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4672-6 PMID: 10758168
  16. Generation of null alleles for the functional analysis of six genes from the right arm of Saccharomyces cerevisiae chromosome II.
    Yeast. 1999 May;15(7):615-23 PMID: 10341424
  17. Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels.
    Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14440-5 PMID: 8962070
  18. Proteome analysis of Saccharomyces cerevisiae: a methodological outline.
    Electrophoresis. 1997 Aug;18(8):1361-72 PMID: 9298650
  19. Genomic disruption of six budding yeast genes gives one drastic example of phenotype strain-dependence.
    Yeast. 1998 May;14(7):655-64 PMID: 9639312
  20. Disruption of six Saccharomyces cerevisiae genes from chromosome IV and basic phenotypic analysis of deletion mutants.
    Yeast. 1998 Sep 30;14(13):1199-208 PMID: 9791891
  21. Two-dimensional gel analysis of the proteome of lager brewing yeasts.
    Yeast. 2000 Apr;16(6):511-22 PMID: 10790688
  22. Filamentous growth in budding yeast.
    Trends Microbiol. 1997 Nov;5(11):450-4 PMID: 9402702
  23. Elevated recombination rates in transcriptionally active DNA.
    Cell. 1989 Feb 24;56(4):619-30 PMID: 2645056
  24. Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach.
    Mol Gen Genet. 1999 Dec;262(4-5):683-702 PMID: 10628851
  25. Characterization of the NHA1 gene encoding a Na+/H+-antiporter of the yeast Saccharomyces cerevisiae.
    FEBS Lett. 1996 May 27;387(1):89-93 PMID: 8654575
  26. Correlation of acidic and basic carrier ampholyte and immobilized pH gradient two-dimensional gel electrophoresis patterns based on mass spectrometric protein identification.
    Electrophoresis. 1998 May;19(6):1024-35 PMID: 9638949
  27. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  28. Disruption of six novel yeast genes located on chromosome II reveals one gene essential for vegetative growth and two required for sporulation and conferring hypersensitivity to various chemicals.
    Yeast. 1999 Jul;15(10B):987-1000 PMID: 10407278
  29. Two-dimensional gel protein database of Saccharomyces cerevisiae.
    Electrophoresis. 1996 Nov;17(11):1683-99 PMID: 8982601
  30. A hyper-recombination mutation in S. cerevisiae identifies a novel eukaryotic topoisomerase.
    Cell. 1989 Jul 28;58(2):409-19 PMID: 2546682
  31. A genetic fine structure analysis of the suppressor 3 locus in Saccharomyces.
    Genetics. 1977 Jan;85(1):55-64 PMID: 320094
  32. The complete sequence of the 8.2 kb segment left of MAT on chromosome III reveals five ORFs, including a gene for a yeast ribokinase.
    Yeast. 1990 Nov-Dec;6(6):521-34 PMID: 1964349
  33. Saccharomyces cerevisiae S288C has a mutation in FLO8, a gene required for filamentous growth.
    Genetics. 1996 Nov;144(3):967-78 PMID: 8913742
  34. Mass spectrometric identification and microcharacterization of proteins from electrophoretic gels: strategies and applications.
    Proteins. 1998;Suppl 2:74-89 PMID: 9849912
  35. Yeast flavohemoglobin is an ancient protein related to globins and a reductase family.
    Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):5015-9 PMID: 1594608
  36. The 1999 SWISS-2DPAGE database update.
    Nucleic Acids Res. 2000 Jan 1;28(1):286-8 PMID: 10592248
  37. Genes affecting the expression of cytochrome c in yeast: genetic mapping and genetic interactions.
    Genetics. 1980 Apr;94(4):871-89 PMID: 6254831
  38. FUS3 represses CLN1 and CLN2 and in concert with KSS1 promotes signal transduction.
    Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9392-6 PMID: 1946350
  39. Aquaporins in Saccharomyces. Genetic and functional distinctions between laboratory and wild-type strains.
    J Biol Chem. 1998 Oct 16;273(42):27565-72 PMID: 9765289
  40. Functional analysis of six genes from chromosomes XIV and XV of Saccharomyces cerevisiae reveals YOR145c as an essential gene and YNL059c/ARP5 as a strain-dependent essential gene encoding nuclear proteins.
    Yeast. 2000 Aug;16(11):1025-33 PMID: 10923024
  41. Yeast proteins related to the p40/laminin receptor precursor are essential components of the 40 S ribosomal subunit.
    J Biol Chem. 1996 May 10;271(19):11383-91 PMID: 8626693
  42. Cloning and nucleotide sequence of the KHS killer gene of Saccharomyces cerevisiae.
    Agric Biol Chem. 1991 Aug;55(8):1953-8 PMID: 1368726
  43. Architectural transcription factors and the SAGA complex function in parallel pathways to activate transcription.
    Mol Cell Biol. 2000 Apr;20(7):2350-7 PMID: 10713159
  44. Two-dimensional electrophoretic separation of yeast proteins using a non-linear wide range (pH 3-10) immobilized pH gradient in the first dimension; reproducibility and evidence for isoelectric focusing of alkaline (pI > 7) proteins.
    Yeast. 1997 Dec;13(16):1519-34 PMID: 9509572
  45. Systematic analysis of yeast strains with possible defects in lipid metabolism.
    Yeast. 1999 May;15(7):601-14 PMID: 10341423
  46. Two distinct domains in the yeast transcription factor IID and evidence for a TATA box-induced conformational change.
    Mol Cell Biol. 1991 Jan;11(1):63-74 PMID: 1986253
Article Info
Journal
Comparative and functional genomics
Abbr.
Comp Funct Genomics
ISSN
1531-6912
DOI
10.1002/cfg.94
Email
ar@cpa.spo.dk
Published
2001-00-00
Pages
207-25
Language
English
Region
Egypt
NLM ID
101090797
PMCID
PMC2447217
External Links
PubMed source DOI: 10.1002/cfg.94 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2