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PMID: 1862107 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation and functional expression of a mammalian prohormone processing enzyme, murine prohormone convertase 1.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 15 ·1991-08-01 ·Pages 6834-8

Korner J, Chun J, Harter D, Axel R

Abstract

We have combined gene cloning with an assay for prohormone biosynthesis and processing in Xenopus oocytes to identify the genes that encode mammalian prohormone processing enzymes. The coinjection of RNA encoding murine prohormone convertase 1 (mPC1), a mammalian endoprotease, along with proopiomelanocortin RNA into an oocyte results in the appropriate cleavage after paired basic residues in the proopiomelanocortin polyprotein necessary to generate corticotropin. The ability of mPC1 to generate corticotropin, along with the observation that mPC1 is specifically expressed in endocrine and neuronal cells, suggests that the mPC1 gene encodes the endopeptidase responsible for the pathway of proopiomelanocortin cleavage observed in the anterior pituitary.

MeSH Terms
Amino Acid Sequence Animals Cell Line Cloning, Molecular Gene Expression Glycosylation Mice Molecular Sequence Data Oocytes/enzymology,physiology Peptide Mapping Pro-Opiomelanocortin/genetics,metabolism Proprotein Convertase 1 Proprotein Convertases Protein Processing, Post-Translational Protein-Tyrosine Kinases Proto-Oncogene Proteins/genetics,isolation & purification,metabolism Proto-Oncogene Proteins c-fes Sequence Homology, Nucleic Acid Serine Endopeptidases/genetics,isolation & purification,metabolism Xenopus laevis
Chemicals
Proto-Oncogene Proteins Pro-Opiomelanocortin Protein-Tyrosine Kinases Fes protein, mouse Proto-Oncogene Proteins c-fes Proprotein Convertases Serine Endopeptidases Pcsk1 protein, mouse Proprotein Convertase 1
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Korner J
Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032.
Chun J
Harter D
Axel R
References (40)
40 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.15.6834
Published
1991-08-01
Pages
6834-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52183
Subset
IM
Grants
NCI NIH HHS · P01CA23767 · United States
Databases
GENBANK
M66858, M66859, M66860, M66861, M66862, M69196, M75099, S45955, S45957, S45959
External Links
PubMed source DOI: 10.1073/pnas.88.15.6834 PMC Full Text View journal details
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