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PMID: 18618654 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

CPEB1 regulates beta-catenin mRNA translation and cell migration in astrocytes.

Glia ·Vol. 56 ·No. 13 ·2008-10-00 ·Pages 1401-13

Jones KJ, Korb E, Kundel MA, Kochanek AR, Kabraji S, McEvoy M, Shin CY, Wells DG

Abstract

A crucial step in directed cell migration is the recruitment of cytoskeletal regulatory and signaling proteins to the leading edge of the cell. One protein localized to the leading edge of a migrating astrocyte is beta-catenin. Using an in vitro wound-healing assay, we show that the localization of beta-catenin to the leading edge is dependent upon new protein synthesis at the time of wounding. We examined the mRNA encoding beta-catenin for potential regulatory elements and identified a conserved cytoplasmic polyadenylation element in the 3'-untranslated region (UTR). We now show that the CPE-binding protein (CPEB1) is expressed in astrocytes and that translation of beta-catenin mRNA is regulated by CPEB1. Further, expression of a mutant CPEB1 protein in astrocytes not only blocks beta-catenin protein localization, it also inhibits cell migration. These findings demonstrate a role for CPEB1-mediated protein synthesis in the localization of beta-catenin protein to the leading edge of migrating astrocytes and in regulating directed cell motility.

MeSH Terms
Animals Astrocytes/cytology,metabolism,physiology Cell Migration Inhibition/physiology Cell Movement/physiology Cells, Cultured Protein Biosynthesis/physiology Rats Transcription Factors/physiology beta Catenin/biosynthesis,genetics mRNA Cleavage and Polyadenylation Factors/physiology
Chemicals
CPEB1 protein, human Transcription Factors beta Catenin mRNA Cleavage and Polyadenylation Factors
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Jones Kendrick J
Department of Molecular Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520-8103, USA.
Korb Erica
Kundel Mitchell A
Kochanek Ashley R
Kabraji Sheheryar
McEvoy Michael
Shin Chan Y
Wells David G
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Article Info
Journal
Glia
Abbr.
Glia
ISSN
1098-1136
DOI
10.1002/glia.20707
Published
2008-10-00
Pages
1401-13
Language
English
Region
United States
NLM ID
8806785
PMCID
PMC3013359
Subset
IM
Grants
NIMH NIH HHS · R01 MH066274 · United States
NIMH NIH HHS · R01 MH066274-05 · United States
NIMH NIH HHS · R01 MH66274 · United States
External Links
PubMed source DOI: 10.1002/glia.20707 PMC Full Text View journal details
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