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PMID: 11702780 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Germ cell differentiation and synaptonemal complex formation are disrupted in CPEB knockout mice.

Developmental cell ·Vol. 1 ·No. 2 ·2001-08-00 ·Pages 201-13

Tay J, Richter JD

Abstract

CPEB is a sequence-specific RNA binding protein that regulates translation during vertebrate oocyte maturation. Adult female CPEB knockout mice contained vestigial ovaries that were devoid of oocytes; ovaries from mid-gestation embryos contained oocytes that were arrested at the pachytene stage. Male CPEB null mice also contained germ cells arrested at pachytene. The germ cells from the knockout mice harbored fragmented chromatin, suggesting a possible defect in homologous chromosome adhesion or synapsis. Two CPE-containing synaptonemal complex protein mRNAs, which interact with CPEB in vitro and in vivo, contained shortened poly(A) tails and mostly failed to sediment with polysomes in the null mice. Synaptonemal complexes were not detected in these animals. CPEB therefore controls germ cell differentiation by regulating the formation of the synaptonemal complex.

MeSH Terms

Age Factors Alleles Animals Apoptosis Cell Differentiation Chromatin/physiology Cytoplasm/metabolism DNA Fragmentation Epididymis/pathology Female Heterozygote Immunohistochemistry In Situ Hybridization In Situ Nick-End Labeling Male Meiosis Mice Mice, Knockout Models, Genetic Ovary/embryology,physiology Poly A Protein Biosynthesis RNA, Messenger/metabolism RNA-Binding Proteins/genetics,metabolism,physiology Reverse Transcriptase Polymerase Chain Reaction Sex Factors Spermatogenesis/genetics,physiology Synaptonemal Complex/physiology Testis/pathology,physiology Time Factors Transcription Factors/genetics,metabolism,physiology Ultraviolet Rays mRNA Cleavage and Polyadenylation Factors

Chemicals

Chromatin Cpeb1 protein, mouse RNA, Messenger RNA-Binding Proteins Transcription Factors mRNA Cleavage and Polyadenylation Factors Poly A

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Tay J

Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655, USA.

Richter J D

Article Info

Journal

Developmental cell

Abbr.

Dev Cell

ISSN

1534-5807

DOI

10.1016/s1534-5807(01)00025-9

Published

2001-08-00

Pages

201-13

Language

English

Region

United States

NLM ID

101120028

Subset

Grants

NICHD NIH HHS · HD07439 · United States

NICHD NIH HHS · HD37267 · United States

External Links

PubMed source DOI: 10.1016/s1534-5807(01)00025-9 View journal details

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Germ cell differentiation and synaptonemal complex formation are disrupted in CPEB knockout mice.

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