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PMID: 1860879 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Yeast cell cycle protein CDC48p shows full-length homology to the mammalian protein VCP and is a member of a protein family involved in secretion, peroxisome formation, and gene expression.

The Journal of cell biology ·Vol. 114 ·No. 3 ·1991-08-00 ·Pages 443-53

Fröhlich KU, Fries HW, Rüdiger M, Erdmann R, Botstein D, Mecke D

Abstract

Yeast mutants of cell cycle gene cdc48-1 arrest as large budded cells with microtubules spreading aberrantly throughout the cytoplasm from a single spindle plaque. The gene was cloned and disruption proved it to be essential. The CDC48 sequence encodes a protein of 92 kD that has an internal duplication of 200 amino acids and includes a nucleotide binding consensus sequence. Vertebrate VCP has a 70% identity over the entire length of the protein. Yeast Sec18p and mammalian N-ethylmaleimide-sensitive fusion protein, which are involved in intracellular transport, yeast Pas1p, which is essential for peroxisome assembly, and mammalian TBP-1, which influences HIV gene expression, are 40% identical in the duplicated region. Antibodies against CDC48 recognize a yeast protein of apparently 115 kD and a mammalian protein of 100 kD. Both proteins are bound loosely to components of the microsomal fraction as described for Sec18p and N-ethylmaleimide-sensitive fusion protein. This similarity suggests that CDC48p participates in a cell cycle function related to that of N-ethylmaleimide-sensitive fusion protein/Sec18p in Golgi transport.

MeSH Terms
Adenosine Triphosphatases Amino Acid Sequence Base Sequence Blotting, Western Cell Cycle Cell Cycle Proteins/genetics Cloning, Molecular DNA, Fungal Fungal Proteins/genetics,immunology,metabolism Gene Expression Histocytochemistry Microbodies/metabolism Molecular Sequence Data Multigene Family Mutation Restriction Mapping Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Alignment Valosin Containing Protein
Chemicals
Cell Cycle Proteins DNA, Fungal Fungal Proteins Saccharomyces cerevisiae Proteins Adenosine Triphosphatases CDC48 protein, S cerevisiae Valosin Containing Protein
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Fröhlich K U
Physiologisch-chemisches Institut der Universität Tübingen, Federal Republic of Germany.
Fries H W
Rüdiger M
Erdmann R
Botstein D
Mecke D
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.114.3.443
Published
1991-08-00
Pages
443-53
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289087
Subset
IM
Databases
GENBANK
M60691, M60692, M60693, M60694, M60695, M60696, M60697, S55286, X56699, X56956
External Links
PubMed source DOI: 10.1083/jcb.114.3.443 PMC Full Text View journal details
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