Novel structure at 5'-ends of nascent DNA chains.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 10 ·1976-10-00 ·Pages 3438-42

Siegmann DW, Werner R

Abstract

Because of their association with protein short nascent DNA chains in Escherichia coli can be separated from other cellular DNA by chromatography on hydroxylapatite. Protein-free DNA chains of less than 500 nucleotides in length are resistant to degradation from the 5'-end by alkaline phosphatase [orthophosphoric-monoester phosphohydrolase (alkaline optimum); EC 3.1.3.1] and spleen phosphodiesterase (oligonucleate 3'-nucleotidohydrolase; EC 3.1.4.18). In contrast, DNA chains containing more than 500 nucleotides are degradable. From these results we conclude that short nascent DNA chains are structurally modified at their 5'-ends. The nature of this structure and its possible functions are discussed.

MeSH Terms

Alkaline Phosphatase/metabolism Animals DNA Replication DNA, Bacterial/analysis,biosynthesis Dogs Molecular Weight Phosphoric Diester Hydrolases/metabolism

Chemicals

DNA, Bacterial Alkaline Phosphatase Phosphoric Diester Hydrolases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Siegmann D W

Werner R

References (16)

16 references, click to expand

Reovirus messenger RNA contains a methylated, blocked 5'-terminal structure: m-7G(5')ppp(5')G-MpCp-.
Proc Natl Acad Sci U S A. 1975 Jan;72(1):362-6 PMID: 1054511
Mechanism of DNA chain growth. IV. Direction of synthesis of T4 short DNA chains as revealed by exonucleolytic degradation.
Proc Natl Acad Sci U S A. 1969 Dec;64(4):1242-8 PMID: 4989398
DNA replication in dnaB mutants of Escherichia coli: gene product interaction and synthesis of 4 S pieces.
J Mol Biol. 1975 Feb 15;92(1):145-63 PMID: 1097700
Synthesis of small polynucleotide chains in thymine-depleted bacteria.
J Mol Biol. 1975 Jun 15;95(1):45-61 PMID: 1097711
Mechanism of DNA chain growth.
J Mol Biol. 1975 Jun 15;95(1):63-70 PMID: 1097712
Mechanism of DNA chain growth. XV. RNA-linked nascent DNA pieces in Escherichia coli strains assayed with spleen exonuclease.
J Mol Biol. 1975 Aug 25;96(4):653-64 PMID: 1104864
On the mechanism of the polynucleotide joining reaction.
Cold Spring Harb Symp Quant Biol. 1968;33:27-34 PMID: 4306816
Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome.
J Mol Biol. 1973 Aug 5;78(2):363-76 PMID: 4355833
Thymine-labelled deoxyoligonucleotide involved in DNA chain growth in Bacillus subtilis.
Nature. 1974 Mar 8;248(5444):147-50 PMID: 4362087
[Catalytic role for RNA in DNA replication].
Nat New Biol. 1972 Mar 29;236(65):101 PMID: 4502801
DNA replication in Escherichia coli: evidence for two classes of small deoxyribonucleotide chains.
J Mol Biol. 1973 Feb 5;73(4):371-96 PMID: 4577039
Mechanism of DNA chain growth. XI. Structure of RNA-linked DNA fragments of Escherichia coli.
J Mol Biol. 1973 Jul 15;77(4):501-17 PMID: 4579449
Mechanism of DNA replication.
Nature. 1971 Apr 30;230(5296):570-2 PMID: 4928662
Nature of DNA precursors.
Nat New Biol. 1971 Sep 22;233(38):99-103 PMID: 4937865
Studies of DNA replication in vivo. I. Isolation of the first intermediate of DNA replication in bacteria as single-stranded DNA.
Proc Natl Acad Sci U S A. 1968 May;60(1):329-36 PMID: 4968634
Novel mutants of Escherichia coli that accumulate very small DNA replicative intermediates.
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2150-4 PMID: 1094455

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.73.10.3438

Published

1976-10-00

Pages

3438-42

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC431130

Subset

External Links

PubMed source DOI: 10.1073/pnas.73.10.3438 PMC Full Text View journal details

Analysis Services

Novel structure at 5'-ends of nascent DNA chains.

Contact

WeChat Official Account