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PMID: 1094455 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Novel mutants of Escherichia coli that accumulate very small DNA replicative intermediates.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 6 ·1975-06-00 ·Pages 2150-4

Konrad EB, Lehman IR

Abstract

A new group of mutants has been isolated which, during short pulses, incorporate (3-H)thymidine into DNA fragments that are substantially smaller than Okazaki fragments. These small fragments can be chased into DNA of high-molecular-weight, and thus may be precursors in DNA replication, During longer pulses, label also appears in DNA of higher molecular weight, although at an abnormally slow rate. The mutations map at a previously undescribed locus (dnaS) at 72 min on the E. coli chromosome.

MeSH Terms
Centrifugation, Density Gradient Chromosome Mapping DNA Nucleotidyltransferases/analysis DNA Replication DNA, Bacterial/biosynthesis Escherichia coli/enzymology,metabolism Exonucleases/analysis Genetics, Microbial Isotope Labeling Mutation Polynucleotide Ligases/analysis Temperature Thymidine/metabolism Time Factors
Chemicals
DNA, Bacterial DNA Nucleotidyltransferases Exonucleases Polynucleotide Ligases Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Konrad E B
Lehman I R
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.6.2150
Published
1975-06-00
Pages
2150-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432714
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.6.2150 PMC Full Text View journal details
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