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PMID: 185365 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

alpha-Adrenergic, beta-adrenergic and cholinergic mechanisms for amylase secretion by rat parotid gland in vitro.

The Journal of physiology ·Vol. 260 ·No. 2 ·1976-09-00 ·Pages 351-70

Leslie BA, Putney JW, Sherman JM

Abstract

1. Rat parotid gland slices, incubated in a balanced, buffered salt solution, were found to be physiologically stable for up to 2 hr with respect to O2 consumption, water content, extracellular space and cation content. 2. The slices could be stimulated to secrete amylase by activation of alpha-adrenergic, beta-adrenergic or muscarinic cholinergic receptors. 3. The secretion elicited through all three receptors appeared to involve exocytosis as revealed by electron microscopy. 4. The beta-agonist, isoprenaline, increased tissue content of cyclic adenosine 3',5'-monophosphate (cyclic AMP); alpha-adrenergic and cholinergic agents had no effect on the level of this cyclic nucleotide. 5. Secretion via cholinergic or alpha-adrenergic mechanisms required extra-cellular calcium; the beta-adrenergic mechanism did not. 6. It was concluded that stimulation of rat parotid cells activates distinctly separate pathways leading ultimately to exocytosis, one pathway involving cyclic AMP, and the other, external Ca2+ ion.

MeSH Terms
Amylases/metabolism Animals Calcium/physiology Cyclic AMP/physiology Exocytosis Extracellular Space/ultrastructure In Vitro Techniques Isoproterenol/pharmacology Male Microscopy, Electron Oxygen Consumption Parotid Gland/drug effects,metabolism,physiology,ultrastructure Rats Receptors, Adrenergic Receptors, Cholinergic
Chemicals
Receptors, Adrenergic Receptors, Cholinergic Cyclic AMP Amylases Isoproterenol Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Leslie B A
Putney J W
Sherman J M
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52 references, click to expand
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1976.sp011519
Published
1976-09-00
Pages
351-70
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1309095
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1976.sp011519 PMC Full Text View journal details
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