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PMID: 4343571 Published · ppublish English Journal Article

Control of pancreatic amylase release in vitro: effects of ions, cyclic AMP, and colchicine.

British journal of pharmacology ·Vol. 46 ·No. 1 ·1972-09-00 ·Pages 66-7

Benz L, Eckstein B, Matthews EK, Williams JA

Abstract

1. The time course and concentration-response relationship of amylase release from pieces of guinea-pig pancreas in vitro in response to bethanechol and pancreozymin was determined.2. Removal of Ca(++) from the medium had no effect on basal amylase release but abolished the stimulating effect on release of bethanechol.3. Elevation of the concentration of Mg(++) in the medium increased basal amylase release and reduced the response to bethanechol.4. Elevation of the concentration of K(+) in the medium increased amylase release; this effect was blocked by a concentration of atropine which blocked also the response to bethanechol.5. Cyclic AMP, dibutyryl cyclic AMP and theophylline failed to stimulate amylase release. Pancreatic cyclic AMP concentrations were found not to be increased by bethanechol, pancreozymin or an elevated concentration of K(+) in the medium.6. Colchicine had no effect on basal amylase release or the response to bethanechol or pancreozymin.7. It is concluded that the coupling of stimulus to secretion involves ionic control but that neither cyclic AMP production nor microtubular mechanisms play a major role in controlling exocytosis in the pancreatic acinar cell. These findings are discussed in relation to the stimulus-secretion coupling processes in other cells.

MeSH Terms
Amylases/metabolism Animals Bethanechol Compounds/pharmacology Calcium/pharmacology Cholecystokinin/pharmacology Colchicine/pharmacology Cyclic AMP/pharmacology Guinea Pigs In Vitro Techniques Magnesium/pharmacology Pancreas/drug effects,enzymology,metabolism Potassium/pharmacology Theophylline/pharmacology
Chemicals
Bethanechol Compounds Cholecystokinin Theophylline Cyclic AMP Amylases Magnesium Potassium Colchicine Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Benz L
Eckstein B
Matthews E K
Williams J A
References (29)
29 references, click to expand
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Article Info
Journal
British journal of pharmacology
Abbr.
Br J Pharmacol
ISSN
0007-1188
DOI
10.1111/j.1476-5381.1972.tb06849.x
Published
1972-09-00
Pages
66-7
Language
English
Region
England
NLM ID
7502536
PMCID
PMC1666108
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1476-5381.1972.tb06849.x PMC Full Text View journal details
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