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PMID: 1852605 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Curved DNA without AA/TT dinucleotide step.

Nucleic acids research ·Vol. 19 ·No. 13 ·1991-07-11 ·Pages 3549-51

Brukner I, Jurukovski V, Konstantinović M, Savić A

Abstract

The evidence is accumulating that dinucleotide steps other than AA/TT affect DNA flexure of AnTm (m + n greater than = 4) containing fragments. However, it is not clear whether macroscopic DNA flexure without AA/TT steps might occur. In this paper we demonstrate the anomaly in electrophoretic mobility of non AA/TT repetitive DNA sequences which is a function of sequence phasing. Therefore, our results show that PyPu (TA) and AG/CT steps, angulary separated by close to 180 degrees from Pu/Py (GC) and GG/CC steps, bend DNA, even in the absence of AnTm tracts.

MeSH Terms
Adenine Nucleotides/chemistry Base Sequence DNA/chemistry Electrophoresis, Polyacrylamide Gel Molecular Sequence Data Nucleic Acid Conformation Nucleotides/chemistry Oligodeoxyribonucleotides/chemistry Repetitive Sequences, Nucleic Acid Thymine Nucleotides/chemistry
Chemicals
Adenine Nucleotides Nucleotides Oligodeoxyribonucleotides Thymine Nucleotides DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Brukner I
Institute of Molecular Genetics and Genetic Engineering, Belgrade, Yugoslavia.
Jurukovski V
Konstantinović M
Savić A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.13.3549
Published
1991-07-11
Pages
3549-51
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328378
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.13.3549 PMC Full Text View journal details
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