Home LiteratureArticle Details
PMID: 1851864 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

E1A represses wild-type and F9-selected polyomavirus DNA replication by a mechanism not requiring depression of large tumor antigen transcription.

Journal of virology ·Vol. 65 ·No. 6 ·1991-06-00 ·Pages 2921-8

DePolo NJ, Villarreal LP

Abstract

Polyomavirus (Py) DNA replication may be regulated to a low-level replication state in specific target cells in mice as well as in certain undifferentiated murine cell lines, such as embryocarcinoma (EC) cells. To investigate possible mechanisms by which such control may occur, we have examined the effects of E1A on Py DNA replication. Adenovirus E1A proteins repress transcriptional activation of various enhancers, including those of Py, and can stimulate DNA replication in quiescent cells, but E1A effects on Py DNA replication were unknown. We found that constitutive E1A expression in NIH 3T3 cells depressed Py DNA replication very strongly. Two F9 EC cell-selected Py enhancer variants, PyF441 and PyF101, were also examined because undifferentiated EC cells are hypothesized to have an E1A-like activity responsible for the Py restriction, and these variants activate Py DNA replication in cis in undifferentiated F9 cells. Both variants were repressed by E1A, indicating that E1A activity in 3T3 cells is not equivalent to undifferentiated F9 cell E1A-like activity. We also examined transient inducible E1A expression in cells supplying Py large tumor antigen (T-Ag). Py DNA replication was again repressed, and the inhibition increased with E1A induction. Analysis of T-Ag mRNA levels indicated that E1A repression of Py DNA replication was not an indirect result of depression of T-Ag transcription. This suggests that E1A may repress Py DNA replication by a more direct mechanism, possibly by blocking enhancer activation of DNA replication in a manner uncoupled with enhancer transcriptional control.

MeSH Terms
Adenovirus Early Proteins Animals Antigens, Viral, Tumor/genetics DNA Replication DNA, Viral/biosynthesis Enhancer Elements, Genetic Gene Expression Regulation, Viral Genetic Variation Oncogene Proteins, Viral/genetics Plasmids Polyomavirus/genetics,immunology RNA, Messenger/metabolism Repressor Proteins/genetics Transfection Tumor Cells, Cultured Virus Replication
Chemicals
Adenovirus Early Proteins Antigens, Viral, Tumor DNA, Viral Oncogene Proteins, Viral RNA, Messenger Repressor Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
DePolo N J
Department of Molecular Biology and Biochemistry, University of California, Irvine 92717.
Villarreal L P
References (64)
64 references, click to expand
  1. An adenovirus early region 1A protein is required for maximal viral DNA replication in growth-arrested human cells.
    J Virol. 1985 Mar;53(3):742-50 PMID: 3973965
  2. Polyomavirus enhancer contains multiple redundant sequence elements that activate both DNA replication and gene expression.
    Mol Cell Biol. 1985 Apr;5(4):649-58 PMID: 2985964
  3. General repression of enhanson activity by the adenovirus-2 E1A proteins.
    Genes Dev. 1990 Jan;4(1):137-50 PMID: 2155159
  4. Detection of DNA and RNA virus genomes in organ systems of whole mice: patterns of mouse organ infection by polyomavirus.
    J Virol. 1984 Jun;50(3):779-83 PMID: 6328007
  5. Polyomavirus growth and persistence in Friend erythroleukemic cells.
    J Virol. 1984 Feb;49(2):566-71 PMID: 6319756
  6. T-antigen-independent replication of polyomavirus DNA in murine embryonal carcinoma cells.
    Mol Cell Biol. 1984 Feb;4(2):317-23 PMID: 6321958
  7. The primary site of replication alters the eventual site of persistent infection by polyomavirus in mice.
    J Virol. 1984 May;50(2):541-6 PMID: 6323753
  8. High efficiency polyoma DNA transfection of chloroquine treated cells.
    Nucleic Acids Res. 1983 Mar 11;11(5):1295-308 PMID: 6298741
  9. The nature of the host range restriction of SV40 and polyoma viruses in embryonal carcinoma cells.
    Curr Top Microbiol Immunol. 1982;101:1-30 PMID: 6303700
  10. An SV40 deletion mutant accumulates late transcripts in a paranuclear extract.
    Virology. 1982 May;119(1):1-11 PMID: 6280378
  11. Polyoma mutants that productively infect F9 embryonal carcinoma cells do not rescue wild-type polyoma in F9 cells.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1479-83 PMID: 6280185
  12. Polyoma DNA sequences involved in control of viral gene expression in murine embryonal carcinoma cells.
    Nature. 1981 Apr 23;290(5808):720-2 PMID: 6261151
  13. Isolation and characterization of polyoma host range mutants that replicate in nullipotential embryonal carcinoma cells.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):1100-4 PMID: 6262755
  14. Mutation near the polyoma DNA replication origin permits productive infection of F9 embryonal carcinoma cells.
    Cell. 1981 Mar;23(3):809-14 PMID: 6261957
  15. Replication of adenovirus and SV40 chromosomes in vitro.
    Philos Trans R Soc Lond B Biol Sci. 1987 Dec 15;317(1187):429-38 PMID: 2894680
  16. Regulation of RNA polymerase III transcription in response to F9 embryonal carcinoma stem cell differentiation.
    Cell. 1989 Dec 22;59(6):1081-92 PMID: 2598261
  17. Insulin gene enhancer activity is inhibited by adenovirus 5 E1a gene products.
    Mol Cell Biol. 1989 Oct;9(10):4531-4 PMID: 2531280
  18. Adenovirus E1A-mediated negative control of genes activated during F9 differentiation.
    Mol Cell Biol. 1989 Jul;9(7):3109-13 PMID: 2528683
  19. An enhancer-like element in the adenovirus E2 promoter contains sequences essential for uninduced and E1A-induced transcription.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):381-5 PMID: 3855557
  20. Cell- and promoter-specific activation of transcription by DNA replication.
    Genes Dev. 1987 Dec;1(10):1065-74 PMID: 2828163
  21. DNA sequence requirements for replication of polyomavirus DNA in vivo and in vitro.
    Mol Cell Biol. 1987 Oct;7(10):3694-704 PMID: 2824994
  22. Mouse p53 inhibits SV40 origin-dependent DNA replication.
    Nature. 1987 Oct 1-7;329(6138):458-60 PMID: 2821401
  23. Negative and positive factors determine the activity of the polyoma virus enhancer alpha domain in undifferentiated and differentiated cell types.
    Proc Natl Acad Sci U S A. 1988 Nov;85(21):7952-6 PMID: 2847148
  24. Activation of the polyomavirus enhancer by a murine activator protein 1 (AP1) homolog and two contiguous proteins.
    Proc Natl Acad Sci U S A. 1988 Aug;85(16):5839-43 PMID: 2842750
  25. Multiple subelements within the polyomavirus enhancer function synergistically to activate DNA replication.
    Mol Cell Biol. 1988 Nov;8(11):5000-15 PMID: 2850472
  26. New host cell system for regulated simian virus 40 DNA replication.
    Mol Cell Biol. 1985 Nov;5(11):3231-40 PMID: 3018509
  27. Negative control of DNA replication in composite SV40-bovine papilloma virus plasmids.
    Cell. 1986 Aug 29;46(5):741-52 PMID: 3017566
  28. A mutated polyoma virus enhancer which is active in undifferentiated embryonal carcinoma cells is not repressed by adenovirus-2 E1A products.
    Nature. 1986 May 15-21;321(6067):249-51 PMID: 3012353
  29. Polyomavirus mutation that confers a cell-specific cis advantage for viral DNA replication.
    Mol Cell Biol. 1985 Aug;5(8):2142-6 PMID: 3018552
  30. A pancreas specificity results from the combination of polyomavirus and Moloney murine leukemia virus enhancer.
    Proc Natl Acad Sci U S A. 1987 Jan;84(2):449-53 PMID: 3025873
  31. Lymphoid and other tissue-specific phenotypes of polyomavirus enhancer recombinants: positive and negative combinational effects on enhancer specificity and activity.
    Mol Cell Biol. 1986 Jun;6(6):2068-79 PMID: 3023917
  32. Adenovirus promoters and E1A transactivation.
    Annu Rev Genet. 1986;20:45-79 PMID: 3028247
  33. Adenovirus E1a proteins repress expression from polyomavirus early and late promoters.
    Mol Cell Biol. 1986 Nov;6(11):4019-25 PMID: 3025625
  34. Selection of mouse neuroblastoma cell-specific polyoma virus mutants with stage differentiative advantages of replication.
    EMBO J. 1985 Dec 1;4(12):3215-21 PMID: 3004942
  35. Induction of a factor that binds to the polyoma virus A enhancer on differentiation of embryonal carcinoma cells.
    Nature. 1987 Jul 16-22;328(6127):254-6 PMID: 3037385
  36. A factor discriminating between the wild-type and a mutant polyomavirus enhancer.
    Nature. 1987 Jul 2-8;328(6125):87-9 PMID: 3037380
  37. Two different factors bind to the alpha-domain of the polyoma virus enhancer, one of which also interacts with the SV40 and c-fos enhancers.
    EMBO J. 1987 May;6(5):1331-7 PMID: 3038517
  38. Developmental control of a promoter-specific factor that is also regulated by the E1A gene product.
    Cell. 1987 Feb 13;48(3):501-6 PMID: 2948654
  39. Multiple functional domains in the adenovirus E1A gene.
    Cell. 1987 Jan 30;48(2):177-8 PMID: 2948653
  40. Repression of insulin gene expression by adenovirus type 5 E1a proteins.
    Mol Cell Biol. 1987 Mar;7(3):1164-70 PMID: 2951590
  41. An adenovirus E1A-like transcription factor is regulated during the differentiation of murine embryonal carcinoma stem cells.
    Cell. 1987 May 22;49(4):507-13 PMID: 2952280
  42. Trans-acting protein factors and the regulation of eukaryotic transcription: lessons from studies on DNA tumor viruses.
    Genes Dev. 1988 Mar;2(3):267-81 PMID: 3288540
  43. Initiation of eukaryotic DNA replication in vitro.
    Annu Rev Cell Biol. 1989;5:197-245 PMID: 2557059
  44. Transcriptional activator nuclear factor I stimulates the replication of SV40 minichromosomes in vivo and in vitro.
    Cell. 1989 Nov 3;59(3):541-51 PMID: 2553273
  45. Multiple transcription factor binding sites mediate adenovirus E1A transactivation.
    J Virol. 1989 Aug;63(8):3499-506 PMID: 2545919
  46. Animal virus DNA replication.
    Annu Rev Biochem. 1989;58:671-717 PMID: 2549858
  47. Transactivation of the adenovirus EIIa promoter in the absence of adenovirus E1A protein is restricted to mouse oocytes and preimplantation embryos.
    Development. 1989 Dec;107(4):945-56 PMID: 2534379
  48. Adenovirus E1A represses transcription of the cellular JE gene.
    J Virol. 1989 Mar;63(3):1470-3 PMID: 2521677
  49. Transcriptional elements as components of eukaryotic origins of DNA replication.
    Cell. 1988 Mar 11;52(5):635-8 PMID: 3278812
  50. Adenovirus-2 E1A products repress enhancer-induced stimulation of transcription.
    Nature. 1984 Dec 13-19;312(5995):608-12 PMID: 6095113
  51. Polyoma virus DNA replication requires an enhancer.
    Nature. 1984 Nov 15-21;312(5991):242-6 PMID: 6095089
  52. p53 and DNA polymerase alpha compete for binding to SV40 T antigen.
    Nature. 1987 Oct 1-7;329(6138):456-8 PMID: 3309672
  53. Individual adenovirus type 5 early region 1A gene products elicit distinct alterations of cellular morphology and gene expression.
    J Virol. 1985 Nov;56(2):404-13 PMID: 4057356
  54. Replicative cis-advantage of polyomavirus regulatory region mutants in different murine cell lines.
    J Virol. 1987 May;61(5):1615-20 PMID: 3033287
  55. Presence of the adenovirus E1A-like activity in preimplantation stage mouse embryos.
    Mol Cell Biol. 1988 Aug;8(8):3553-5 PMID: 2974925
  56. Adenovirus E1a proteins repress transcription from the SV40 early promoter.
    Cell. 1985 Mar;40(3):705-16 PMID: 2982503
  57. Adenovirus E1A products suppress myogenic differentiation and inhibit transcription from muscle-specific promoters.
    Nature. 1988 Apr 7;332(6164):553-7 PMID: 2965790
  58. Simian virus 40 replication in adenovirus-transformed human cells antagonizes gene expression.
    Nature. 1985 Sep 12-18;317(6033):169-71 PMID: 2993921
  59. Host species specificity of polyomavirus DNA replication is not altered by simian virus 40 72-base-pair repeats.
    Mol Cell Biol. 1985 Jun;5(6):1534-7 PMID: 2993870
  60. Functions of adenovirus E1A.
    Cancer Surv. 1986;5(2):367-87 PMID: 2946406
  61. Common control of the heat shock gene and early adenovirus genes: evidence for a cellular E1A-like activity.
    Mol Cell Biol. 1984 May;4(5):867-74 PMID: 6547205
  62. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  63. The repression of the growth factor-inducible genes JE, c-myc and stromelysin by adenovirus E1A is mediated by conserved region 1.
    Oncogene. 1989 Oct;4(10):1207-12 PMID: 2529466
  64. Minimal subenhancer requirements for high-level polyomavirus DNA replication: a cell-specific synergy of PEA3 and PEA1 sites.
    Mol Cell Biol. 1990 Sep;10(9):4996-5001 PMID: 2167444
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.6.2921-2928.1991
Published
1991-06-00
Pages
2921-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240926
Subset
IM
Grants
NIGMS NIH HHS · GM 36605 · United States
NIGMS NIH HHS · GM1117702 · United States
External Links
PubMed source DOI: 10.1128/JVI.65.6.2921-2928.1991 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2