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PMID: 1849267 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Drosophila P-element transposase is a transcriptional repressor in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 7 ·1991-04-01 ·Pages 2613-7

Kaufman PD, Rio DC

Abstract

Mobility of P transposable elements in Drosophila melanogaster depends on the 87-kDa transposase protein encoded by the P element. Transposase recognizes a 10-base-pair DNA sequence that overlaps an A + T-rich region essential for transcription from the P-element promoter. We report here that transposase represses transcription from the P-element promoter in vitro. This transcriptional repression is blocked by prior formation of an RNA polymerase II transcription complex on the template DNA. Binding of transposase on the P-element promoter is blocked by prior binding of either the Drosophila RNA polymerase II complex or the yeast transcription factor TFIID. These data suggest that transposase represses transcription by preventing assembly of an RNA polymerase II complex at the P-element promoter.

MeSH Terms
Animals Base Sequence Binding Sites DNA Transposable Elements Drosophila melanogaster/enzymology,genetics Molecular Sequence Data Nucleotidyltransferases/metabolism Promoter Regions, Genetic RNA Polymerase II/metabolism Repressor Proteins/metabolism TATA Box Transcription, Genetic Transposases
Chemicals
DNA Transposable Elements Repressor Proteins Nucleotidyltransferases RNA Polymerase II Transposases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kaufman P D
Whitehead Institute for Biomedical Research, Nine Cambridge Center, MA 02142.
Rio D C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.7.2613
Published
1991-04-01
Pages
2613-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51288
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.7.2613 PMC Full Text View journal details
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