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PMID: 1848707 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Murine erythroleukemia cell line GM979 contains factors that can activate silent chromosomal human gamma-globin genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 6 ·1991-03-15 ·Pages 2530-4

Zitnik G, Hines P, Stamatoyannopoulos G, Papayannopoulou T

Abstract

We introduced a normal chromosome 11 into GM979 murine erythroleukemia cells by fusing them with Epstein-Barr virus-transformed lymphocytes from a normal individual. In contrast to previous data obtained with other murine erythroleukemia cells, we detected activation of human chromosomal gamma-globin genes in GM979 cells. GM979, unlike previously used murine erythroleukemia cell lines, expresses murine embryonic globin in addition to adult globin. While all the hybrids expressed gamma- and beta-globin, they displayed a wide range of gamma-globin expression in relation to that of beta-globin. No correlation, however, was found in quantitative expression between murine embryonic globin and human gamma-globin in these hybrids, suggesting that the two globins are regulated independently, at least in this cell line. These data indicate that gamma-globin genes from normal, nonerythroid chromosomes are not irreversibly silenced, and they can be activated by a positive trans factor(s) present in GM979 cells.

MeSH Terms
Animals Cell Line Cell Transformation, Viral Chromosomes, Human, Pair 11 Clone Cells Gene Expression Regulation Globins/genetics Herpesvirus 4, Human/genetics Humans Leukemia, Erythroblastic, Acute Leukemia, Experimental Mice Transfection
Chemicals
Globins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Zitnik G
Division of Medical Genetics, University of Washington, Seattle 98195.
Hines P
Stamatoyannopoulos G
Papayannopoulou T
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.6.2530
Published
1991-03-15
Pages
2530-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51266
Subset
IM
Grants
NIDDK NIH HHS · DK30852 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.6.2530 PMC Full Text View journal details
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