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PMID: 1848648 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Detection and abundance of mRNA and protein encoded by transposable element activator (Ac) in maize.

Molecular & general genetics : MGG ·Vol. 225 ·No. 2 ·1991-02-00 ·Pages 186-92

Fusswinkel H, Schein S, Courage U, Starlinger P, Kunze R

Abstract

The 3.5 kb long mRNA of the maize transposable element Ac contains an open reading frame (ORFa) which encodes a polypeptide of 807 amino acids, the putative transposase of Ac. The Ac mRNA is a rare transcript: we now estimate the fraction of Ac mRNA in wx-m7::Ac seedlings to be 2-13 x 10(-5) of the polyA RNA. Assuming that maize cells contain similar amounts of polyA RNA as another monocot (0.16 pg/cell), this is equivalent to 1.5-10 transcripts in each cell. A protein with an apparent molecular weight of 112 kDa is detected, by five antisera directed against different segments of ORFa, exclusively in nuclear extracts from Ac-containing maize. This protein is most likely the full-length Ac ORFa protein. We estimate its concentration to be in the range of 3 x 10(-7) of the nuclear proteins, or about 1000 molecules per triploid endosperm cell containing one Ac element.

MeSH Terms
Blotting, Northern Blotting, Western DNA Transposable Elements Molecular Weight Nucleotidyltransferases/genetics,metabolism Open Reading Frames RNA, Messenger/analysis,genetics Restriction Mapping Transcription, Genetic Transposases Zea mays/enzymology,genetics
Chemicals
DNA Transposable Elements RNA, Messenger Nucleotidyltransferases Transposases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fusswinkel H
Institut für Genetik, Universität zu Köln, Federal Republic of Germany.
Schein S
Courage U
Starlinger P
Kunze R
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00269846
Published
1991-02-00
Pages
186-92
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00269846
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