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PMID: 1846964 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

cAMP stimulation of acetylcholine receptor expression is mediated through posttranslational mechanisms.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 3 ·1991-02-01 ·Pages 854-8

Green WN, Ross AF, Claudio T

Abstract

When the four Torpedo acetylcholine receptor (AcChoR) subunit cDNAs are stably integrated into the genome of mouse fibroblast cells, alpha 2 beta gamma delta pentamers with proper pharmacological and electrophysiological properties are expressed on the cell surface. Expression of these AcChoRs can be regulated by agents that stimulate intracellular cAMP levels with the result that increased numbers of cell-surface AcChoRs are produced. Theophylline, 8-(4-chlorophenylthio)-adenosine 3':5'-cyclic monophosphate, cholera toxin, and forskolin stimulated AcChoR cell-surface expression 1.2-, 1.6-, 2.2-, and 2.3-fold, respectively. cAMP-stimulated expression is mediated through a posttranslational mechanism, and the observed increase in surface AcChoRs correlates with increased lifetimes of each newly synthesized subunit. Increased subunit lifetimes are not observed in cell lines expressing each subunit individually, indicating that subunit stabilization arises through heterologous subunit-subunit interactions.

MeSH Terms
Animals Bungarotoxins/metabolism Cell Line Cell Membrane/metabolism Cholera Toxin/pharmacology Colforsin/pharmacology Cyclic AMP/analogs & derivatives,pharmacology,physiology Cycloheximide/pharmacology DNA/genetics Kinetics L Cells/metabolism Macromolecular Substances Mice Protein Processing, Post-Translational/drug effects Receptors, Cholinergic/drug effects,genetics Theophylline/pharmacology Thionucleotides/pharmacology Torpedo Transfection
Chemicals
Bungarotoxins Macromolecular Substances Receptors, Cholinergic Thionucleotides Colforsin 8-((4-chlorophenyl)thio)cyclic-3',5'-AMP DNA Cholera Toxin Cycloheximide Theophylline Cyclic AMP
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Green W N
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510.
Ross A F
Claudio T
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28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.3.854
Published
1991-02-01
Pages
854-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50912
Subset
IM
Grants
NHLBI NIH HHS · HL38156 · United States
NINDS NIH HHS · NS07102 · United States
NINDS NIH HHS · NS21714 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.3.854 PMC Full Text View journal details
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