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PMID: 184468 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Loss of mouse chromosomes in somatic cell hybrids between HT-1080 human fibrosarcoma cells and mouse peritioneal macrophages.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 9 ·1976-09-00 ·Pages 3248-52

Croce CM

Abstract

Somatic cell hybrids between mouse peritioneal macrophages and HT-1080 human fibrosarcoma cells lose mouse chromosomes and retain the entire complement of human chromosomes. In contrast, somatic cell hybrids between cells derived from two different mouse continuous cell lines and HT-1080 human cells were found to lose human chromosomes preferentially. Loss of mouse chromosomes is not a general property of hybrids between mouse macrophages and transformed human cells; the hybridization of mouse macrophages with cells derived from five different human fibroblast lines transformed by simian virus 40 resulted in the production of hybrid clones that preferentially lost human chromosomes.

MeSH Terms
Animals Ascitic Fluid/cytology Carbohydrate Epimerases/analysis Cell Line Cell Transformation, Neoplastic Chromosomes/ultrastructure Fibrosarcoma/genetics Glucuronidase/analysis Humans Hybrid Cells/enzymology,ultrastructure Isoenzymes/analysis Karyotyping Macrophages/ultrastructure Mice Mice, Inbred Strains Simian virus 40
Chemicals
Isoenzymes Glucuronidase Carbohydrate Epimerases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Croce C M
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.9.3248
Published
1976-09-00
Pages
3248-52
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430994
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.9.3248 PMC Full Text View journal details
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