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PMID: 169524 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Assignment of gene(s) for cell transformation to human chromosome 7 carrying the simian virus 40 genome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 5 ·1975-05-00 ·Pages 1658-60

Croce CM, Koprowski H

Abstract

Somatic cell hybrid clones between either C57BL/6 or Balb/c mouse peritoneal macrophages and two different simian virus 40 (SV40)-transformed human cell lines deficient in hypoxanthine phosphoribosyltransferase (EC 2.4.2.8; IMP:pyrophosphate phosphoribosyltransferase) were obtained in hypoxanthine-aminopterin-thymidine selective medium. All the hybrid cell clones contained the human chromosome 7, which carries the SV40 genome, and were SV40 tumor (T)-antigen positive. No hybrid cell clones studied displayed the density-dependent inhibition of cell growth characteristic of normal cells; all clones had a high saturation density and gave origin to cell colonies when plated in soft agar. Since the expression of the transformed phenotype was always associated with the presence of the human chromosome 7, which carries the SV40 genome, it is concluded that this chromosome contains gene(s) [Tr gene(s)] coding for "transforming factor(s)."

MeSH Terms
Animals Antigens Cell Division Cell Fusion Cell Transformation, Neoplastic Cerebral Hemorrhage/diagnosis Chromosomes, Human, 6-12 and X/metabolism Genes Genotype Humans Hybrid Cells Hypoxanthine Phosphoribosyltransferase/metabolism Karyotyping Macrophages/metabolism Mice Mice, Inbred BALB C Phenotype Simian virus 40/immunology,metabolism
Chemicals
Antigens Hypoxanthine Phosphoribosyltransferase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Croce C M
Koprowski H
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17 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.5.1658
Published
1975-05-00
Pages
1658-60
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432602
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.5.1658 PMC Full Text View journal details
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