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PMID: 18420932 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The chemical genomic portrait of yeast: uncovering a phenotype for all genes.

Science (New York, N.Y.) ·Vol. 320 ·No. 5874 ·2008-04-18 ·Pages 362-5

Hillenmeyer ME, Fung E, Wildenhain J, Pierce SE, Hoon S, Lee W, Proctor M, St Onge RP, Tyers M, Koller D, Altman RB, Davis RW, Nislow C, Giaever G

Abstract

Genetics aims to understand the relation between genotype and phenotype. However, because complete deletion of most yeast genes ( approximately 80%) has no obvious phenotypic consequence in rich medium, it is difficult to study their functions. To uncover phenotypes for this nonessential fraction of the genome, we performed 1144 chemical genomic assays on the yeast whole-genome heterozygous and homozygous deletion collections and quantified the growth fitness of each deletion strain in the presence of chemical or environmental stress conditions. We found that 97% of gene deletions exhibited a measurable growth phenotype, suggesting that nearly all genes are essential for optimal growth in at least one condition.

MeSH Terms
Culture Media Drug Resistance, Multiple, Fungal Gene Deletion Genes, Essential Genes, Fungal Genes, MDR Genome, Fungal Genomics Heterozygote Homozygote Metabolic Networks and Pathways/drug effects Multigene Family Phenotype Saccharomyces cerevisiae/drug effects,genetics,growth & development,physiology Saccharomyces cerevisiae Proteins/genetics,metabolism Signal Transduction Small Molecule Libraries/pharmacology
Chemicals
Culture Media Saccharomyces cerevisiae Proteins Small Molecule Libraries
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Hillenmeyer Maureen E
Stanford Genome Technology Center, Stanford University, Palo Alto, CA 94304, USA.
Fung Eula
Wildenhain Jan
Pierce Sarah E
Hoon Shawn
Lee William
Proctor Michael
St Onge Robert P
Tyers Mike
Koller Daphne
Altman Russ B
Davis Ronald W
Nislow Corey
Giaever Guri
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Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
DOI
10.1126/science.1150021
Published
2008-04-18
Pages
362-5
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC2794835
Subset
IM
Grants
NIGMS NIH HHS · U01 GM061374 · United States
NIGMS NIH HHS · U01 GM061374-09 · United States
External Links
PubMed source DOI: 10.1126/science.1150021 PMC Full Text View journal details
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