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PMID: 1839359 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Up-regulation of the integrin alpha 1/beta 1 in human neuroblastoma cells differentiated by retinoic acid: correlation with increased neurite outgrowth response to laminin.

Cell regulation ·Vol. 2 ·No. 12 ·1991-12-00 ·Pages 1021-33

Rossino P, Defilippi P, Silengo L, Tarone G

Abstract

Retinoic acid (RA) is known to induce differentiation of neuroblastoma cells in vitro. Here we show that treatment of two human neuroblastoma cell lines, SY5Y and IMR32, with RA resulted in a fivefold increase of the integrin alpha 1/beta 1 expression. The effect was selective because expression of the alpha 3/beta 1 integrin, also present in these cells, was not increased. The up-regulation of the alpha 1/beta 1 differentiated SY5Y cells correlated with increased neurite response to laminin. In fact, RA-treated SY5Y cells elongated neurites on laminin-coated substratum more efficiently compared with untreated cells or cells treated with nerve growth factor, insulin, or phorbol 12-myristate 13-acetate. These three agents induced partial morphological differentiation but did not increase alpha 1 integrin expression. Neurite extension in RA-treated cells was more efficient on laminin than on fibronectin or collagen type I and was inhibited with beta 1 integrin antibodies on all three substrates. Affinity chromatography experiments showed that alpha 1/beta 1 is the major laminin receptor in both untreated and RA-treated SY5Y cells. These data show that RA, a naturally occurring morphogen implicated in embryonic development, can selectively regulate the expression of integrin complexes in neuronal cells and suggest an important role of the alpha 1/beta 1 laminin receptor in the morphological differentiation of nerve cells.

MeSH Terms
Cell Differentiation/drug effects Humans Integrins/metabolism Laminin/pharmacology Neurites/drug effects Neuroblastoma/immunology,metabolism,pathology Neurons/cytology,drug effects,metabolism Receptors, Immunologic/metabolism Receptors, Laminin Tretinoin/pharmacology Tumor Cells, Cultured/drug effects,metabolism,pathology Up-Regulation
Chemicals
Integrins Laminin Receptors, Immunologic Receptors, Laminin Tretinoin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rossino P
Department of Genetics, Biology and Medical Chemistry, University of Torino, Italy.
Defilippi P
Silengo L
Tarone G
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Article Info
Journal
Cell regulation
Abbr.
Cell Regul
ISSN
1044-2030
DOI
10.1091/mbc.2.12.1021
Published
1991-12-00
Pages
1021-33
Language
English
Region
United States
NLM ID
9005331
PMCID
PMC361902
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.2.12.1021 PMC Full Text
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