Home LiteratureArticle Details
PMID: 18390672 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of the RND-type multidrug efflux pump MexAB-OprM of the plant pathogen Pseudomonas syringae.

Applied and environmental microbiology ·Vol. 74 ·No. 11 ·2008-06-00 ·Pages 3387-93

Stoitsova SO, Braun Y, Ullrich MS, Weingart H

Abstract

In gram-negative bacteria, transporters belonging to the RND family are the transporters most relevant for resistance to antimicrobial compounds. In Pseudomonas aeruginosa, a clinically important pathogen, the RND-type pump MexAB-OprM has been recognized as one of the major multidrug efflux systems. Here, homologues of MexAB-OprM in the plant pathogens Pseudomonas syringae pv. phaseolicola 1448A, P. syringae pv. syringae B728a, and P. syringae pv. tomato DC3000 were identified, and mexAB-oprM-deficient mutants were generated. Determination of MICs revealed that mutation of MexAB-OprM dramatically reduced the tolerance to a broad range of antimicrobials. Moreover, the ability of the mexAB-oprM-deficient mutants to multiply in planta was reduced. RNA dot blot hybridization revealed growth-dependent regulation of the mexAB-oprM operon in P. syringae; the expression of this operon was maximal in early exponential phase and decreased gradually during further growth.

MeSH Terms
Anti-Bacterial Agents/pharmacology Arabidopsis/microbiology Bacterial Proteins/genetics Biological Transport, Active/genetics Drug Resistance, Multiple, Bacterial/genetics Ethidium/metabolism Gene Deletion Gene Expression Profiling Membrane Transport Proteins/genetics Microbial Sensitivity Tests Multidrug Resistance-Associated Proteins/genetics Phaseolus/microbiology Pseudomonas syringae/drug effects,genetics,growth & development,metabolism
Chemicals
Anti-Bacterial Agents Bacterial Proteins Membrane Transport Proteins Multidrug Resistance-Associated Proteins Ethidium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stoitsova Savina O
School of Engineering and Science, Jacobs University Bremen, Bremen, Germany.
Braun Yvonne
Ullrich Matthias S
Weingart Helge
References (37)
37 references, click to expand
  1. Multiresistance genes of Rhizobium etli CFN42.
    Mol Plant Microbe Interact. 2000 May;13(5):572-7 PMID: 10796024
  2. Antibiotic-dependent induction of Pseudomonas putida DOT-T1E TtgABC efflux pump is mediated by the drug binding repressor TtgR.
    Antimicrob Agents Chemother. 2003 Oct;47(10):3067-72 PMID: 14506010
  3. Two simple media for the demonstration of pyocyanin and fluorescin.
    J Lab Clin Med. 1954 Aug;44(2):301-7 PMID: 13184240
  4. PHYTOALEXINS: What Have We Learned After 60 Years?
    Annu Rev Phytopathol. 1999;37:285-306 PMID: 11701825
  5. Enhancement of the mexAB-oprM efflux pump expression by a quorum-sensing autoinducer and its cancellation by a regulator, MexT, of the mexEF-oprN efflux pump operon in Pseudomonas aeruginosa.
    Antimicrob Agents Chemother. 2004 Apr;48(4):1320-8 PMID: 15047536
  6. The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000.
    Proc Natl Acad Sci U S A. 2003 Sep 2;100(18):10181-6 PMID: 12928499
  7. A DHA14 drug efflux gene from Xanthomonas albilineans confers high-level albicidin antibiotic resistance in Escherichia coli.
    J Appl Microbiol. 2006 Jul;101(1):151-60 PMID: 16834602
  8. Identification and characterization of the emhABC efflux system for polycyclic aromatic hydrocarbons in Pseudomonas fluorescens cLP6a.
    J Bacteriol. 2003 Nov;185(21):6233-40 PMID: 14563857
  9. Characterization of a resistance-nodulation-cell division transporter system associated with the syr-syp genomic island of Pseudomonas syringae pv. syringae.
    Appl Environ Microbiol. 2005 Sep;71(9):5056-65 PMID: 16151087
  10. The MexA-MexB-OprM multidrug efflux system of Pseudomonas aeruginosa is growth-phase regulated.
    FEMS Microbiol Lett. 1999 Apr 1;173(1):35-9 PMID: 10220878
  11. Extraction of high-quality bacterial RNA from infected leaf tissue for bacterial in planta gene expression analysis by multiplexed fluorescent Northern hybridization.
    Mol Plant Pathol. 2008 Mar;9(2):227-35 PMID: 18705854
  12. Genetic locus encoding functions involved in biosynthesis and outer membrane localization of xanthomonadin in Xanthomonas oryzae pv. oryzae.
    J Bacteriol. 2002 Jul;184(13):3539-48 PMID: 12057948
  13. Antibiotic efflux pumps in prokaryotic cells: occurrence, impact on resistance and strategies for the future of antimicrobial therapy.
    J Antimicrob Chemother. 2003 May;51(5):1055-65 PMID: 12697642
  14. Multidrug efflux systems play an important role in the invasiveness of Pseudomonas aeruginosa.
    J Exp Med. 2002 Jul 1;196(1):109-18 PMID: 12093875
  15. Stress-Induced Phenylpropanoid Metabolism.
    Plant Cell. 1995 Jul;7(7):1085-1097 PMID: 12242399
  16. The phytoalexin-inducible multidrug efflux pump AcrAB contributes to virulence in the fire blight pathogen, Erwinia amylovora.
    Mol Plant Microbe Interact. 2004 Jan;17(1):43-54 PMID: 14714867
  17. Bacterial resistance to antibiotics: active efflux and reduced uptake.
    Adv Drug Deliv Rev. 2005 Jul 29;57(10):1486-513 PMID: 15939505
  18. Nucleotide sequence analysis of a transposon (Tn5393) carrying streptomycin resistance genes in Erwinia amylovora and other gram-negative bacteria.
    J Bacteriol. 1993 Feb;175(3):732-40 PMID: 8380801
  19. An isoflavonoid-inducible efflux pump in Agrobacterium tumefaciens is involved in competitive colonization of roots.
    J Bacteriol. 1998 Jun;180(12):3107-13 PMID: 9620959
  20. Bacteria in the leaf ecosystem with emphasis on Pseudomonas syringae-a pathogen, ice nucleus, and epiphyte.
    Microbiol Mol Biol Rev. 2000 Sep;64(3):624-53 PMID: 10974129
  21. Efflux-mediated multiresistance in Gram-negative bacteria.
    Clin Microbiol Infect. 2004 Jan;10(1):12-26 PMID: 14706082
  22. The role of several multidrug resistance systems in Erwinia chrysanthemi pathogenesis.
    Mol Plant Microbe Interact. 2006 Jun;19(6):607-13 PMID: 16776294
  23. Molecular mechanisms that confer antibacterial drug resistance.
    Nature. 2000 Aug 17;406(6797):775-81 PMID: 10963607
  24. NorM, an Erwinia amylovora multidrug efflux pump involved in in vitro competition with other epiphytic bacteria.
    Appl Environ Microbiol. 2004 Feb;70(2):693-703 PMID: 14766544
  25. Expression of the multidrug resistance operon mexA-mexB-oprM in Pseudomonas aeruginosa: mexR encodes a regulator of operon expression.
    Antimicrob Agents Chemother. 1996 Sep;40(9):2021-8 PMID: 8878574
  26. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648-52 PMID: 377280
  27. Multidrug pump inhibitors uncover remarkable activity of plant antimicrobials.
    Antimicrob Agents Chemother. 2002 Oct;46(10):3133-41 PMID: 12234835
  28. Use of fluorescence probes to monitor function of the subunit proteins of the MexA-MexB-oprM drug extrusion machinery in Pseudomonas aeruginosa.
    J Biol Chem. 1997 Aug 29;272(35):21964-9 PMID: 9268332
  29. Evolution of secondary metabolites from an ecological and molecular phylogenetic perspective.
    Phytochemistry. 2003 Sep;64(1):3-19 PMID: 12946402
  30. Multiple antibiotic resistance in Pseudomonas aeruginosa: evidence for involvement of an efflux operon.
    J Bacteriol. 1993 Nov;175(22):7363-72 PMID: 8226684
  31. Erwinia chrysanthemi tolC is involved in resistance to antimicrobial plant chemicals and is essential for phytopathogenesis.
    J Bacteriol. 2003 Oct;185(19):5772-8 PMID: 13129948
  32. A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants.
    Gene. 1998 May 28;212(1):77-86 PMID: 9661666
  33. Multidrug efflux pumps and resistance: regulation and evolution.
    Curr Opin Microbiol. 2003 Oct;6(5):446-51 PMID: 14572535
  34. Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000.
    Proc Natl Acad Sci U S A. 2005 Aug 2;102(31):11064-9 PMID: 16043691
  35. Effects of Environmental and Nutritional Factors on Production of the Polyketide Phytotoxin Coronatine by Pseudomonas syringae pv. Glycinea.
    Appl Environ Microbiol. 1993 May;59(5):1619-26 PMID: 16348941
  36. Whole-genome sequence analysis of Pseudomonas syringae pv. phaseolicola 1448A reveals divergence among pathovars in genes involved in virulence and transposition.
    J Bacteriol. 2005 Sep;187(18):6488-98 PMID: 16159782
  37. A quorum-sensing autoinducer enhances the mexAB-oprM efflux-pump expression without the MexR-mediated regulation in Pseudomonas aeruginosa.
    Microbiol Immunol. 2004;48(5):435-9 PMID: 15215631
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
1098-5336
DOI
10.1128/AEM.02866-07
Published
2008-06-00
Epub
2008-00-04
Pages
3387-93
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC2423046
Subset
IM
External Links
PubMed source DOI: 10.1128/AEM.02866-07 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2