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PMID: 18385338 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Subventricular zone-mediated ependyma repair in the adult mammalian brain.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 28 ·No. 14 ·2008-04-02 ·Pages 3804-13

Luo J, Shook BA, Daniels SB, Conover JC

Abstract

The subventricular zone (SVZ) of the adult mouse brain is a narrow stem cell niche that lies along the length of the lateral wall of the lateral ventricles. The SVZ supports neurogenesis throughout adulthood; however, with increasing age, the ventral SVZ deteriorates and only the dorsolateral SVZ remains neurogenic. Associated with the elderly dorsolateral SVZ, we reported previously an increased number of astrocytes interposed within the adjacent ependymal lining. Here, we show that astrocytes integrated within the ependyma are dividing, BrdU-labeled astrocytes that share cellular adherens with neighboring ependymal cells. By tracking BrdU-labeled astrocytes over time, we observed that, as they incorporated within the ependyma, they took on antigenic and morphologic characteristics of ependymal cells, suggesting a novel form of SVZ-supported "regenerative" repair in the aging brain. A similar form of SVZ-mediated ependyma repair was also observed in young mice after mild ependymal cell denudation with low dosages of neuraminidase. Together, this work identifies a novel non-neuronal mechanism of regenerative repair by the adult SVZ.

MeSH Terms
Adult Stem Cells/physiology,ultrastructure Age Factors Aging/pathology Animals Astrocytes/physiology,ultrastructure Brain/anatomy & histology Bromodeoxyuridine/metabolism Cell Count/methods Dose-Response Relationship, Drug Ependyma/drug effects,injuries,physiopathology,ultrastructure Lateral Ventricles/cytology,ultrastructure Male Mice Microscopy, Confocal/methods Microscopy, Electron/methods Nerve Tissue Proteins/metabolism Neuraminidase/adverse effects
Chemicals
Nerve Tissue Proteins Neuraminidase Bromodeoxyuridine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Luo Jie
Center for Regenerative Biology, Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut 06269-4243, USA.
Shook Brett A
Daniels Stephen B
Conover Joanne C
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.0224-08.2008
Published
2008-04-02
Pages
3804-13
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC2638095
Subset
IM
Grants
NINDS NIH HHS · R01 NS050338 · United States
NINDS NIH HHS · R01 NS050338-02 · United States
NINDS NIH HHS · NS45894 · United States
NINDS NIH HHS · NS050338 · United States
External Links
PubMed source DOI: 10.1523/JNEUROSCI.0224-08.2008 PMC Full Text View journal details
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