The ATPase activity of RecA is needed to push the DNA strand exchange through heterologous regions.
The role of ATP hydrolysis during the RecA-mediated recombination reaction is addressed in this paper. Recent studies indicated that the RecA-promoted DNA strand exchange between completely homologous double- and single-stranded DNA can be very efficient in the absence of ATP hydrolysis. In this work we demonstrate that the energy derived from the ATP hydrolysis is strictly needed to drive the DNA strand exchange through the regions where the interacting DNA molecules are not in a homologous register. Therefore, in addition to the role of the ATP hydrolysis in promoting the dissociation of RecA from the products of the recombination reaction, as described earlier, ATP hydrolysis also plays a crucial role in the actual process of strand exchange, provided that the lack of homologous register obstructs the process of branch migration.
17 references, click to expand
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Biochemistry of genetic recombination: energetics and mechanism of DNA strand exchange.Annu Rev Biophys Biophys Chem. 1991;20:539-75 PMID: 1831022
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Physical map of the recA gene.Proc Natl Acad Sci U S A. 1979 Jul;76(7):3144-8 PMID: 386332
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The RecA protein: structure and function.Crit Rev Biochem Mol Biol. 1990;25(6):415-56 PMID: 2292186
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Networks of DNA and RecA protein are intermediates in homologous pairing.Biochemistry. 1985 Jun 18;24(13):3226-32 PMID: 3161539
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The contrasting structures of mismatched DNA sequences containing looped-out bases (bulges) and multiple mismatches (bubbles).Nucleic Acids Res. 1989 Sep 12;17(17):6821-40 PMID: 2780311
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Visualization of RecA-DNA complexes involved in consecutive stages of an in vitro strand exchange reaction.Cold Spring Harb Symp Quant Biol. 1984;49:561-70 PMID: 6397311
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Electron microscopic visualization of the RecA protein-mediated pairing and branch migration phases of DNA strand exchange.J Biol Chem. 1987 Sep 15;262(26):12812-20 PMID: 3305514
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Visualization of the paranemic joining of homologous DNA molecules catalyzed by the RecA protein of Escherichia coli.Proc Natl Acad Sci U S A. 1986 Apr;83(7):2066-70 PMID: 3515345
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The formation of plectonemic joints by the recA protein of Escherichia coli. Requirement for ATP hydrolysis.J Biol Chem. 1985 Jan 10;260(1):170-3 PMID: 3880737
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The pairing activity of stable nucleoprotein filaments made from recA protein, single-stranded DNA, and adenosine 5'-(gamma-thio)triphosphate.J Biol Chem. 1985 Sep 25;260(21):11845-51 PMID: 3840165
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Stable DNA heteroduplex formation catalyzed by the Escherichia coli RecA protein in the absence of ATP hydrolysis.Proc Natl Acad Sci U S A. 1990 Jan;87(1):21-5 PMID: 2404275
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Characterization of the DNA binding activity of stable RecA-DNA complexes. Interaction between the two DNA binding sites within RecA helical filaments.J Mol Biol. 1990 Mar 5;212(1):97-112 PMID: 2319601
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Insertions, deletions and mismatches in heteroduplex DNA made by recA protein.Cell. 1983 Dec;35(2 Pt 1):511-20 PMID: 6317195
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Role of RecA protein spiral filaments in genetic recombination.Nature. 1984 May 17-23;309(5965):215-9 PMID: 6325943
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recA protein of Escherichia coli promotes branch migration, a kinetically distinct phase of DNA strand exchange.Proc Natl Acad Sci U S A. 1981 Jun;78(6):3433-7 PMID: 7022448
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A simple and rapid procedure for the large scale purification of the recA protein of Escherichia coli.J Biol Chem. 1981 May 10;256(9):4676-8 PMID: 7012155
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RecA-DNA helical filaments in genetic recombination.Biochimie. 1991 Feb-Mar;73(2-3):199-208 PMID: 1883882
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