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PMID: 18354040 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Global characterization of cell-specific gene expression through fluorescence-activated sorting of nuclei.

Plant physiology ·Vol. 147 ·No. 1 ·2008-05-00 ·Pages 30-40

Zhang C, Barthelson RA, Lambert GM, Galbraith DW

Abstract

We describe a simple and highly effective means for global identification of genes that are expressed within specific cell types within complex tissues. It involves transgenic expression of nuclear-targeted green fluorescent protein in a cell-type-specific manner. The fluorescent nuclei are then purified from homogenates by fluorescence-activated sorting, and the RNAs employed as targets for microarray hybridization. We demonstrate the validity of the approach through the identification of 12 genes that are selectively expressed in phloem.

MeSH Terms
Arabidopsis/genetics,metabolism Cell Nucleus/metabolism Flow Cytometry/methods Gene Expression Genes, Plant Green Fluorescent Proteins/metabolism Phloem/metabolism Plants, Genetically Modified/metabolism Promoter Regions, Genetic Protoplasts/metabolism
Chemicals
Green Fluorescent Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Zhang Changqing
Department of Plant Sciences , The University of Arizona, Tucson, AZ 85721, USA.
Barthelson Roger A
Lambert Georgina M
Galbraith David W
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.107.115246
Published
2008-05-00
Epub
2008-00-19
Pages
30-40
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC2330299
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.107.115246 PMC Full Text View journal details
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