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PMID: 18342603 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Transcription factor substitution during the evolution of fungal ribosome regulation.

Molecular cell ·Vol. 29 ·No. 5 ·2008-03-14 ·Pages 552-62

Hogues H, Lavoie H, Sellam A, Mangos M, Roemer T, Purisima E, Nantel A, Whiteway M

Abstract

Coordinated ribosomal protein (RP) gene expression is crucial for cellular viability, but the transcriptional network controlling this regulon has only been well characterized in the yeast Saccharomyces cerevisiae. We have used whole-genome transcriptional and location profiling to establish that, in Candida albicans, the RP regulon is controlled by the Myb domain protein Tbf1 working in conjunction with Cbf1. These two factors bind both the promoters of RP genes and the rDNA locus; Tbf1 activates transcription at these loci and is essential. Orthologs of Tbf1 bind TTAGGG telomeric repeats in most eukaryotes, and TTAGGG cis-elements are present upstream of RP genes in plants and fungi, suggesting that Tbf1 was involved in both functions in ancestral eukaryotes. In all Hemiascomycetes, Rap1 substituted Tbf1 at telomeres and, in the S. cerevisiae lineage, this substitution also occurred independently at RP genes, illustrating the extreme adaptability and flexibility of transcriptional regulatory networks.

MeSH Terms
Base Sequence Candida albicans/genetics,metabolism Computational Biology DNA, Ribosomal/metabolism DNA-Binding Proteins/genetics,metabolism Evolution, Molecular Fungal Proteins/genetics,metabolism Gene Expression Profiling Gene Expression Regulation, Fungal Genome, Fungal Molecular Sequence Data Promoter Regions, Genetic Regulon Ribosomes/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Shelterin Complex Telomere/metabolism Telomere-Binding Proteins/genetics,metabolism Transcription Factors/genetics,metabolism
Chemicals
CBF1 protein, Candida albicans DNA, Ribosomal DNA-Binding Proteins Fungal Proteins RAP1 protein, Candida albicans RAP1 protein, S cerevisiae Saccharomyces cerevisiae Proteins Shelterin Complex TBF1 protein, S cerevisiae Telomere-Binding Proteins Transcription Factors
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Hogues Hervé
Biotechnology Research Institute, National Research Council, Montreal, QC H4P 2R2, Canada.
Lavoie Hugo
Sellam Adnane
Mangos Maria
Roemer Terry
Purisima Enrico
Nantel André
Whiteway Malcolm
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Article Info
Journal
Molecular cell
Abbr.
Mol Cell
ISSN
1097-4164
DOI
10.1016/j.molcel.2008.02.006
Published
2008-03-14
Pages
552-62
Language
English
Region
United States
NLM ID
9802571
PMCID
PMC3838363
Subset
IM
Corrections
CommentIn
PMID: 18342598
CommentIn
PMID: 18342600
External Links
PubMed source DOI: 10.1016/j.molcel.2008.02.006 PMC Full Text View journal details
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