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PMID: 1833773 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Affinity purification of Trypanosoma brucei small nuclear ribonucleoproteins reveals common and specific protein components.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 20 ·1991-10-15 ·Pages 9097-101

Palfi Z, Günzl A, Cross M, Bindereif A

Abstract

We have developed a procedure for the affinity purification of small nuclear ribonucleoproteins (snRNPs) of Trypanosoma brucei (U2 and U4/U6 snRNPs), which are essential for trans splicing. Each of these snRNPs can be specifically and efficiently selected from T. brucei extracts through biotinylated antisense 2'-O-methylated RNA oligonucleotides immobilized on streptavidin-agarose. Protein analysis revealed a set of five low molecular weight polypeptides common to the U2 and U4/U6 snRNPs and the spliced leader RNP. In addition, several U2 and U4/U6 snRNP-specific protein components were identified. Using monoclonal antibodies against human snRNP proteins, we could not detect any significant cross-reaction with the trypanosomal U2 snRNP proteins. Thus, the trypanosomal snRNPs exhibit principal differences from the higher eukaryotic snRNPs not only in their RNA but also in their protein components.

MeSH Terms
Animals Antibodies, Monoclonal Base Sequence Cross Reactions Humans Immunoblotting Molecular Sequence Data Nucleic Acid Conformation Oligonucleotides, Antisense RNA Splicing RNA, Small Nuclear/genetics,isolation & purification Ribonucleoproteins/immunology,isolation & purification,metabolism Ribonucleoproteins, Small Nuclear Trypanosoma brucei brucei/genetics,metabolism
Chemicals
Antibodies, Monoclonal Oligonucleotides, Antisense RNA, Small Nuclear Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Palfi Z
Max-Planck-Institut für Molekulare Genetik, Otto-Warburg-Laboratorium, Federal Republic of Germany.
Günzl A
Cross M
Bindereif A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.20.9097
Published
1991-10-15
Pages
9097-101
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52659
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.20.9097 PMC Full Text View journal details
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