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PMID: 18310344 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of novel genes and pathways affecting Salmonella type III secretion system 1 using a contact-dependent hemolysis assay.

Journal of bacteriology ·Vol. 190 ·No. 9 ·2008-05-00 ·Pages 3393-8

Field TR, Layton AN, Bispham J, Stevens MP, Galyov EE

Abstract

We screened 5,700 Salmonella enterica serovar Typhimurium mutants for defects in type III secretion system 1 (T3SS-1)-mediated contact-dependent hemolysis to identify novel genes and pathways affecting the activity of T3SS-1. Our data suggest that previously unrecognized factors such as type I fimbriae may modulate the expression, activity, or deployment of this key virulence factor.

MeSH Terms
Bacterial Proteins/genetics Biological Assay DNA-Binding Proteins/genetics Fimbriae, Bacterial/genetics Genes, Bacterial Hemolysis Mutation Protein Transport/genetics Salmonella typhimurium/genetics,pathogenicity Virulence Factors/genetics
Chemicals
Bacterial Proteins DNA-Binding Proteins FimW protein, Salmonella enterica Spi1 protein, Salmonella Virulence Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Field Terry R
Division of Microbiology, Institute for Animal Health, Compton, Berkshire, RG20 7NN, United Kingdom.
Layton Abigail N
Bispham Jennie
Stevens Mark P
Galyov Edouard E
References (26)
26 references, click to expand
  1. FimW is a negative regulator affecting type 1 fimbrial expression in Salmonella enterica serovar typhimurium.
    J Bacteriol. 2001 Jan;183(2):435-42 PMID: 11133935
  2. Adhesin-dependent binding and uptake of Salmonella enterica serovar Typhimurium by dendritic cells.
    Microbiology (Reading). 2007 Apr;153(Pt 4):1059-1069 PMID: 17379714
  3. Type III secretion-dependent hemolytic activity of enteropathogenic Escherichia coli.
    Infect Immun. 1999 Oct;67(10):5538-40 PMID: 10496946
  4. IpaB of Shigella flexneri causes entry into epithelial cells and escape from the phagocytic vacuole.
    EMBO J. 1992 May;11(5):1991-9 PMID: 1582426
  5. Characterization of Salmonella pathogenicity island 1 type III secretion-dependent hemolytic activity in Salmonella enterica serovar Typhimurium.
    Microb Pathog. 2004 Aug;37(2):65-72 PMID: 15312846
  6. Mutation of waaN reduces Salmonella enterica serovar Typhimurium-induced enteritis and net secretion of type III secretion system 1-dependent proteins.
    Infect Immun. 2000 Jun;68(6):3768-71 PMID: 10816545
  7. Transcriptional activation of Salmonella typhimurium invasion genes by a member of the phosphorylated response-regulator superfamily.
    Mol Microbiol. 1996 Nov;22(4):715-27 PMID: 8951818
  8. Differential binding to and biofilm formation on, HEp-2 cells by Salmonella enterica serovar Typhimurium is dependent upon allelic variation in the fimH gene of the fim gene cluster.
    Mol Microbiol. 2002 Sep;45(5):1255-65 PMID: 12207694
  9. Protein delivery into eukaryotic cells by type III secretion machines.
    Nature. 2006 Nov 30;444(7119):567-73 PMID: 17136086
  10. BopB is a type III secreted protein in Bordetella bronchiseptica and is required for cytotoxicity against cultured mammalian cells.
    Cell Microbiol. 2003 Dec;5(12):973-83 PMID: 14641181
  11. Construction and characterization of type 1 non-fimbriate and non-adhesive mutants of Salmonella typhimurium.
    FEMS Immunol Med Microbiol. 1997 Dec;19(4):289-96 PMID: 9537754
  12. Contribution of fimbrial operons to attachment to and invasion of epithelial cell lines by Salmonella typhimurium.
    Infect Immun. 1996 May;64(5):1862-5 PMID: 8613405
  13. Type 1 fimbriae of Salmonella enterica serovar Typhimurium bind to enterocytes and contribute to colonization of swine in vivo.
    Infect Immun. 2003 Nov;71(11):6446-52 PMID: 14573666
  14. SopE, a secreted protein of Salmonella dublin, is translocated into the target eukaryotic cell via a sip-dependent mechanism and promotes bacterial entry.
    Mol Microbiol. 1996 Oct;22(2):327-38 PMID: 8930917
  15. Multiple factors independently regulate hilA and invasion gene expression in Salmonella enterica serovar typhimurium.
    J Bacteriol. 2000 Apr;182(7):1872-82 PMID: 10714991
  16. The YopB protein of Yersinia pseudotuberculosis is essential for the translocation of Yop effector proteins across the target cell plasma membrane and displays a contact-dependent membrane disrupting activity.
    EMBO J. 1996 Nov 1;15(21):5812-23 PMID: 8918459
  17. Molecular basis for the enterocyte tropism exhibited by Salmonella typhimurium type 1 fimbriae.
    J Biol Chem. 1999 Feb 26;274(9):5797-809 PMID: 10026202
  18. Bacterial injectisomes: needle length does matter.
    Science. 2005 Feb 25;307(5713):1278 PMID: 15731447
  19. Mechanisms of Salmonella entry into host cells.
    Cell Microbiol. 2007 Sep;9(9):2103-11 PMID: 17593246
  20. Optimization of virulence functions through glucosylation of Shigella LPS.
    Science. 2005 Feb 25;307(5713):1313-7 PMID: 15731456
  21. Molecular basis of Salmonella-induced enteritis.
    Mol Microbiol. 2000 Jun;36(5):997-1005 PMID: 10844685
  22. Flagellin A is essential for the virulence of Vibrio anguillarum.
    J Bacteriol. 1996 Mar;178(5):1310-9 PMID: 8631707
  23. Analysis of type 1 fimbriae expression in verotoxigenic Escherichia coli: a comparison between serotypes O157 and O26.
    Microbiology (Reading). 2001 Jan;147(Pt 1):145-52 PMID: 11160808
  24. Transmembrane movement of exogenous long-chain fatty acids: proteins, enzymes, and vectorial esterification.
    Microbiol Mol Biol Rev. 2003 Sep;67(3):454-72, table of contents PMID: 12966144
  25. Manipulating cellular transport and immune responses: dynamic interactions between intracellular Salmonella enterica and its host cells.
    Cell Microbiol. 2006 May;8(5):728-37 PMID: 16611223
  26. Identification of host-specific colonization factors of Salmonella enterica serovar Typhimurium.
    Mol Microbiol. 2004 Nov;54(4):994-1010 PMID: 15522082
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
1098-5530
DOI
10.1128/JB.01189-07
Published
2008-05-00
Epub
2008-00-29
Pages
3393-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC2347372
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BBS/E/I/00001021 · United Kingdom
Biotechnology and Biological Sciences Research Council · D010632/1 · United Kingdom
External Links
PubMed source DOI: 10.1128/JB.01189-07 PMC Full Text View journal details
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