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PMID: 1830649 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genetic depletion indicates a late role for U5 snRNP during in vitro spliceosome assembly.

Nucleic acids research ·Vol. 19 ·No. 14 ·1991-07-25 ·Pages 3857-60

Séraphin B, Abovich N, Rosbash M

Abstract

The pre-mRNA splicing pathway is highly conserved from yeast (S. cerevisiae) to mammals. Of the four snRNPs involved in splicing three (U1, U2 and U4/U6) have been shown to be essential for in vitro splicing. To examine the remaining snRNP, we utilized our previously described genetic procedures (Seraphin and Rosbash, 1989) to prepare yeast extracts depleted of U5 snRNP. The results show that U5 snRNP is necessary for both steps of pre- mRNA splicing and for proper spliceosome assembly, i.e., addition of the U4/U5/U6 triple snRNP. The prior steps of U1 and U2 snRNP addition occur normally in the absence of U5 snRNP.

Related Genes
U5
MeSH Terms
Blotting, Northern Centrifugation Genetic Complementation Test RNA Precursors/metabolism RNA Splicing RNA, Fungal/metabolism Ribonucleoproteins/genetics,physiology Ribonucleoproteins, Small Nuclear Saccharomyces cerevisiae/genetics
Chemicals
RNA Precursors RNA, Fungal Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Séraphin B
Howard Hughes Medical Institute, Department of Biology, Brandeis University, Waltham, MA 02254.
Abovich N
Rosbash M
References (34)
34 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.14.3857
Published
1991-07-25
Pages
3857-60
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328474
Subset
IM
Grants
NIGMS NIH HHS · GM23549 · United States
External Links
PubMed source DOI: 10.1093/nar/19.14.3857 PMC Full Text View journal details
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