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PMID: 1828808 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transfer of glycosyl-phosphatidylinositol membrane anchors to polypeptide acceptors in a cell-free system.

The Journal of cell biology ·Vol. 114 ·No. 1 ·1991-07-00 ·Pages 61-71

Mayor S, Menon AK, Cross GA

Abstract

Glycosylinositol phospholipid (GPI) membrane anchors are the sole means of membrane attachment of a large number of cell surface proteins, including the variant surface glycoproteins (VSGs) of the parasitic protozoan, Trypanosoma brucei. Biosynthetic data suggest that GPI-anchored proteins are synthesized with carboxy-terminal extensions that are immediately replaced by GPI, suggesting the existence of preformed GPI species available for transfer to the nascent protein in the ER. Candidate precursor glycolipids having a linear sequence indistinguishable from the conserved core structure found on all GPI anchors, have been characterized in T. brucei. In this paper we describe the transfer of three GPI variants to endogenous VSG in vitro. GPI addition is not reduced by inhibitors of protein synthesis and does not require ATP or GTP, consistent with a transpeptidation mechanism.

MeSH Terms
Adenosine Triphosphate/metabolism Animals Cell-Free System Endoplasmic Reticulum/metabolism Glycolipids/metabolism Glycosylation Glycosylphosphatidylinositols Guanosine Triphosphate/metabolism Phosphatidylinositols/metabolism Protein Biosynthesis Trypanosoma brucei brucei Variant Surface Glycoproteins, Trypanosoma/metabolism
Chemicals
Glycolipids Glycosylphosphatidylinositols Phosphatidylinositols Variant Surface Glycoproteins, Trypanosoma Guanosine Triphosphate Adenosine Triphosphate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mayor S
Rockefeller University, New York, New York 10021.
Menon A K
Cross G A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.114.1.61
Published
1991-07-00
Pages
61-71
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289052
Subset
IM
Grants
NIAID NIH HHS · AI21531 · United States
External Links
PubMed source DOI: 10.1083/jcb.114.1.61 PMC Full Text View journal details
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