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PMID: 3858818 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Rapid processing of the carboxyl terminus of a trypanosome variant surface glycoprotein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 10 ·1985-05-00 ·Pages 3207-11

Bangs JD, Hereld D, Krakow JL, Hart GW, Englund PT

Abstract

The variant surface glycoprotein of the parasite Trypanosoma brucei contains a glycolipid of unknown structure covalently attached to its COOH terminus. We have shown, by using metabolic labeling with [35S]methionine or [3H]myristic acid, precipitation with specific antibodies, and NaDodSO4/polyacrylamide gel electrophoresis, that this glycolipid is attached to the variant surface glycoprotein polypeptide within 1 min after its translation.

MeSH Terms
Amino Acid Sequence Animals Glycolipids/metabolism Glycoproteins/immunology,metabolism Kinetics Membrane Proteins/immunology,metabolism Molecular Weight Myristic Acid Myristic Acids/metabolism Protein Processing, Post-Translational Trypanosoma brucei brucei/immunology,metabolism Tunicamycin/pharmacology Variant Surface Glycoproteins, Trypanosoma
Chemicals
Glycolipids Glycoproteins Membrane Proteins Myristic Acids Variant Surface Glycoproteins, Trypanosoma Myristic Acid Tunicamycin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bangs J D
Hereld D
Krakow J L
Hart G W
Englund P T
References (30)
30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.10.3207
Published
1985-05-00
Pages
3207-11
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397744
Subset
IM
Grants
NIAID NIH HHS · AI21334-01 · United States
NIGMS NIH HHS · GM-07445 · United States
NIGMS NIH HHS · GM7309 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.10.3207 PMC Full Text View journal details
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