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PMID: 18287006 Published · ppublish English Journal Article Research Support, N.I.H., Intramural Research Support, Non-U.S. Gov't

Gating at the selectivity filter in cyclic nucleotide-gated channels.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 105 ·No. 9 ·2008-03-04 ·Pages 3310-4

Contreras JE, Srikumar D, Holmgren M

Abstract

By opening and closing the permeation pathway (gating) in response to cGMP binding, cyclic nucleotide-gated (CNG) channels serve key roles in the transduction of visual and olfactory signals. Compiling evidence suggests that the activation gate in CNG channels is not located at the intracellular end of pore, as it has been established for voltage-activated potassium (K(V)) channels. Here, we show that ion permeation in CNG channels is tightly regulated at the selectivity filter. By scanning the entire selectivity filter using small cysteine reagents, like cadmium and silver, we observed a state-dependent accessibility pattern consistent with gated access at the middle of the selectivity filter, likely at the corresponding position known to regulate structural changes in KcsA channels in response to low concentrations of permeant ions.

MeSH Terms
Amino Acid Substitution Animals Cadmium Cell Line Cyclic GMP Cyclic Nucleotide-Gated Cation Channels/chemistry,genetics,metabolism Cysteine Ion Channel Gating/genetics Protein Conformation Silver Substrate Specificity Transfection
Chemicals
Cyclic Nucleotide-Gated Cation Channels Cadmium Silver Cyclic GMP Cysteine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Contreras Jorge E
Porter Neuroscience Research Center, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892-3701, USA.
Srikumar Deepa
Holmgren Miguel
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.0709809105
Published
2008-03-04
Epub
2008-00-20
Pages
3310-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2265121
Subset
IM
Grants
Intramural NIH HHS · United States
External Links
PubMed source DOI: 10.1073/pnas.0709809105 PMC Full Text View journal details
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