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PMID: 18281434 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Characterization of the archaeal community in a minerotrophic fen and terminal restriction fragment length polymorphism-directed isolation of a novel hydrogenotrophic methanogen.

Applied and environmental microbiology ·Vol. 74 ·No. 7 ·2008-04-00 ·Pages 2059-68

Cadillo-Quiroz H, Yashiro E, Yavitt JB, Zinder SH

Abstract

Minerotrophic fen peatlands are widely distributed in northern latitudes and, because of their rapid turnover of organic matter, are potentially larger sources of atmospheric methane than bog peatlands per unit area. However, studies of the archaeal community composition in fens are scarce particularly in minerotrophic sites. Several 16S rRNA-based primer sets were used to obtain a broad characterization of the archaeal community in a minerotrophic fen in central New York State. A wide archaeal diversity was observed in the site: 11 euryarchaeal and 2 crenarchaeal groups, most of which were uncultured. The E1 group, a novel cluster in the order Methanomicrobiales, and Methanosaetaceae were the codominant groups in all libraries and results of terminal restriction fragment length polymorphism (T-RFLP) analysis. Given its abundance and potential hydrogenotrophic methane contribution, the E1 group was targeted for culture attempts with a low-ionic-strength medium (PM1). Initial attempts yielded Methanospirillum-dominated cultures. However, by incorporating a T-RFLP analysis as a quick selection tool for treatments and replicates, we were able to select an enrichment dominated by E1. Further dilutions to 10(-9) and tracking with T-RFLP yielded a strain named E1-9c. E1-9c is a novel coccoid hydrogenotrophic, mesophilic, slightly acidophilic methanogen and is highly sensitive to Na(2)S concentrations (requires <0.12 mM for growth). We propose E1-9c as the first representative of a novel genus in the Methanomicrobiales order.

MeSH Terms
Archaea DNA, Archaeal/analysis DNA, Ribosomal Methane/metabolism Methanomicrobiales/genetics,isolation & purification,metabolism Molecular Sequence Data Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S/analysis,genetics Soil/analysis Soil Microbiology
Chemicals
DNA, Archaeal DNA, Ribosomal RNA, Ribosomal, 16S Soil Methane
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cadillo-Quiroz Hinsby
Department of Microbiology, Cornell University, 270 Wing Hall, Ithaca, NY 14853, USA.
Yashiro Erica
Yavitt Joseph B
Zinder Stephen H
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
1098-5336
DOI
10.1128/AEM.02222-07
Published
2008-04-00
Epub
2008-00-15
Pages
2059-68
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC2292581
Subset
IM
Databases
GENBANK
EU155896, EU155897, EU155898, EU155899, EU155900, EU155901, EU155902, EU155903, EU155904, EU155905, EU155906, EU155907, EU155908, EU155909, EU155910, EU155911, EU155912, EU155913, EU155914, EU155915, EU155916, EU155917, EU155918, EU155919, EU155920, EU155921, EU155922, EU155923, EU155924, EU155925, EU155926, EU155927, EU155928, EU155929, EU155930, EU155931, EU155932, EU155933, EU155934, EU155935, EU155936, EU155937, EU155938, EU155939, EU155940, EU155941, EU155942, EU155943, EU155944, EU155945, EU155946, EU155947, EU155948, EU155949, EU155950, EU155951, EU155952, EU155953, EU155954, EU155955, EU155956, EU155957, EU155958, EU155959, EU155960, EU155961, EU155962, EU155963, EU155964, EU155965, EU155966, EU155967, EU155968, EU155969, EU155970, EU155971, EU155972, EU155973, EU155974, EU155975, EU155976, EU155977, EU155978, EU155979, EU155980, EU155981, EU155982, EU155983, EU155984, EU155985, EU155986, EU155987, EU155988, EU155989, EU155990, EU155991, EU155992, EU155993, EU155994, EU155995, EU155996, EU155997, EU155998, EU155999, EU156000
External Links
PubMed source DOI: 10.1128/AEM.02222-07 PMC Full Text View journal details
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