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PMID: 18165328 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The downy mildew effector proteins ATR1 and ATR13 promote disease susceptibility in Arabidopsis thaliana.

The Plant cell ·Vol. 19 ·No. 12 ·2007-12-00 ·Pages 4077-90

Sohn KH, Lei R, Nemri A, Jones JD

Abstract

The downy mildew (Hyaloperonospora parasitica) effector proteins ATR1 and ATR13 trigger RPP1-Nd/WsB- and RPP13-Nd-dependent resistance, respectively, in Arabidopsis thaliana. To better understand the functions of these effectors during compatible and incompatible interactions of H. parasitica isolates on Arabidopsis accessions, we developed a novel delivery system using Pseudomonas syringae type III secretion via fusions of ATRs to the N terminus of the P. syringae effector protein, AvrRPS4. ATR1 and ATR13 both triggered the hypersensitive response (HR) and resistance to bacterial pathogens in Arabidopsis carrying RPP1-Nd/WsB or RPP13-Nd, respectively, when delivered from P. syringae pv tomato (Pst) DC3000. In addition, multiple alleles of ATR1 and ATR13 confer enhanced virulence to Pst DC3000 on susceptible Arabidopsis accessions. We conclude that ATR1 and ATR13 positively contribute to pathogen virulence inside host cells. Two ATR13 alleles suppressed bacterial PAMP (for Pathogen-Associated Molecular Patterns)-triggered callose deposition in susceptible Arabidopsis when delivered by DC3000 DeltaCEL mutants. Furthermore, expression of another allele of ATR13 in plant cells suppressed PAMP-triggered reactive oxygen species production in addition to callose deposition. Intriguingly, although Wassilewskija (Ws-0) is highly susceptible to H. parasitica isolate Emco5, ATR13Emco5 when delivered by Pst DC3000 triggered localized immunity, including HR, on Ws-0. We suggest that an additional H. parasitica Emco5 effector might suppress ATR13-triggered immunity.

MeSH Terms
Alleles Amino Acid Motifs/genetics Arabidopsis/genetics,metabolism,microbiology Arabidopsis Proteins/genetics,metabolism Bacterial Proteins/genetics,metabolism Electrophoresis, Polyacrylamide Gel Gene Expression Regulation, Plant Glucans/metabolism Immunity, Innate Molecular Sequence Data Plant Diseases/genetics,microbiology Plant Leaves/genetics,metabolism,microbiology Polymerase Chain Reaction Proto-Oncogene Proteins c-myb/genetics,metabolism Pseudomonas syringae/genetics,pathogenicity Reactive Oxygen Species/metabolism Virulence/genetics
Chemicals
ATR1 protein, Arabidopsis Arabidopsis Proteins AvrRps4 protein, Pseudomonas syringae Bacterial Proteins Glucans Proto-Oncogene Proteins c-myb Reactive Oxygen Species avrRpt2 protein, Pseudomonas syringae callose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sohn Kee Hoon
Sainsbury Laboratory, John Ines Centre, Norwich NR4 7UH, United Kingdom.
Lei Rita
Nemri Adnane
Jones Jonathan D G
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.107.054262
Published
2007-12-00
Epub
2007-00-28
Pages
4077-90
Language
English
Region
England
NLM ID
9208688
PMCID
PMC2217653
Subset
IM
Databases
GENBANK
AJ870977, AY785302, AY785305, AY842877, AY842883, L43559
External Links
PubMed source DOI: 10.1105/tpc.107.054262 PMC Full Text View journal details
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