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PMID: 17941710 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

A comprehensive genetic characterization of bacterial motility.

PLoS genetics ·Vol. 3 ·No. 9 ·2007-09-00 ·Pages 1644-60

Girgis HS, Liu Y, Ryu WS, Tavazoie S

Abstract

We have developed a powerful experimental framework that combines competitive selection and microarray-based genetic footprinting to comprehensively reveal the genetic basis of bacterial behaviors. Application of this method to Escherichia coli motility identifies 95% of the known flagellar and chemotaxis genes, and reveals three dozen novel loci that, to varying degrees and through diverse mechanisms, affect motility. To probe the network context in which these genes function, we developed a method that uncovers genome-wide epistatic interactions through comprehensive analyses of double-mutant phenotypes. This allows us to place the novel genes within the context of signaling and regulatory networks, including the Rcs phosphorelay pathway and the cyclic di-GMP second-messenger system. This unifying framework enables sensitive and comprehensive genetic characterization of complex behaviors across the microbial biosphere.

MeSH Terms
Bacillus subtilis/genetics,physiology Chemotaxis Epistasis, Genetic Escherichia coli/genetics,physiology Genome, Bacterial Second Messenger Systems Signal Transduction
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Girgis Hany S
Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA.
Liu Yirchung
Ryu William S
Tavazoie Saeed
Conflict of Interest

Competing interests. The authors have declared that no competing interests exist.

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Article Info
Journal
PLoS genetics
Abbr.
PLoS Genet
ISSN
1553-7404
DOI
10.1371/journal.pgen.0030154
Published
2007-09-00
Epub
2007-00-25
Pages
1644-60
Language
English
Region
United States
NLM ID
101239074
PMCID
PMC1976333
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pgen.0030154 PMC Full Text View journal details
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