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PMID: 17924117 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The involvement of a P38-like MAP kinase in ABA-induced and H2O2-mediated stomatal closure in Vicia faba L.

Plant cell reports ·Vol. 27 ·No. 2 ·2008-02-00 ·Pages 377-85

Jiang J, Wang P, An G, Wang P, Song CP

Abstract

SB203580 is a specific inhibitor of p38 mitogen-activated protein (MAP) kinase and has been widely used to investigate the physiological roles of p38 in animal and yeast cells. Here by using an epidermal strip bioassay, laser-scanning confocal microscopy and whole-cell patch clamp analysis, we assess the effects of pyridinyl imidazoles-like SB203580 on the H(2)O(2) signaling in guard cells of Vicia faba L. The results indicated that SB203580 blocks H(2)O(2)- or ABA-induced stomatal closure, ABA-induced H(2)O(2) generation, and decrease in K(+) fluxing across plasma membrane of Vicia guard cells by application of ABA and H(2)O(2), whereas its analog SB202474 had no effect on these events. Thus, these results suggest that activation of p38-like MAP kinase modulates guard cell ROS signaling in response to stress.

MeSH Terms
Abscisic Acid/pharmacology Hydrogen Peroxide/metabolism Imidazoles/pharmacology Microscopy, Confocal Plant Epidermis/cytology,metabolism Plant Proteins/metabolism Signal Transduction/drug effects Vicia faba/cytology,drug effects,metabolism p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors,metabolism
Chemicals
Imidazoles Plant Proteins SB202494 Abscisic Acid Hydrogen Peroxide p38 Mitogen-Activated Protein Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Jiang Jing
Laboratory of Plant Stress Biology and College of Life Sciences, Henan University, Kaifeng, Henan 475001, People's Republic of China. jiangjing@henu.edu.cn
Wang Pengtao
An Guoyong
Wang Pengcheng
Song Chun-Peng
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Article Info
Journal
Plant cell reports
Abbr.
Plant Cell Rep
ISSN
0721-7714
DOI
10.1007/s00299-007-0449-x
Email
jiangjing@henu.edu.cn
Published
2008-02-00
Epub
2007-00-09
Pages
377-85
Language
English
Region
Germany
NLM ID
9880970
Subset
IM
External Links
PubMed source DOI: 10.1007/s00299-007-0449-x View journal details
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