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PMID: 17893323 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dynamic interactions of Ntr1-Ntr2 with Prp43 and with U5 govern the recruitment of Prp43 to mediate spliceosome disassembly.

Molecular and cellular biology ·Vol. 27 ·No. 23 ·2007-12-00 ·Pages 8027-37

Tsai RT, Tseng CK, Lee PJ, Chen HC, Fu RH, Chang KJ, Yeh FL, Cheng SC

Abstract

The Saccharomyces cerevisiae splicing factors Ntr1 (also known as Spp382) and Ntr2 form a stable complex and can further associate with DExD/H-box RNA helicase Prp43 to form a functional complex, termed the NTR complex, which catalyzes spliceosome disassembly. We show that Prp43 interacts with Ntr1-Ntr2 in a dynamic manner. The Ntr1-Ntr2 complex can also bind to the spliceosome first, before recruiting Prp43 to catalyze disassembly. Binding of Ntr1-Ntr2 or Prp43 does not require ATP, but disassembly of the spliceosome requires hydrolysis of ATP. The NTR complex also dynamically interacts with U5 snRNP. Ntr2 interacts with U5 component Brr2 and is essential for both interactions of NTR with U5 and with the spliceosome. Ntr2 alone can also bind to U5 and to the spliceosome, suggesting a role of Ntr2 in mediating the binding of NTR to the spliceosome through its interaction with U5. Our results demonstrate that dynamic interactions of NTR with U5, through the interaction of Ntr2 with Brr2, and interactions of Ntr1 and Prp43 govern the recruitment of Prp43 to the spliceosome to mediate spliceosome disassembly.

MeSH Terms
Adenosine Triphosphate/pharmacology Antibodies, Fungal/pharmacology DEAD-box RNA Helicases/metabolism Protein Binding/drug effects Ribonucleoprotein, U5 Small Nuclear/metabolism Saccharomyces cerevisiae/drug effects,metabolism Saccharomyces cerevisiae Proteins/metabolism Spliceosomes/drug effects,metabolism
Chemicals
Antibodies, Fungal Ntr2 protein, S cerevisiae Ribonucleoprotein, U5 Small Nuclear Saccharomyces cerevisiae Proteins Spp382 protein, S cerevisiae Adenosine Triphosphate PRP43 protein, S cerevisiae DEAD-box RNA Helicases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Tsai Rong-Tzong
Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, Taiwan, Republic of China.
Tseng Chi-Kang
Lee Pei-Jung
Chen Hsin-Chou
Fu Ru-Huei
Chang Kae-jiun
Yeh Fu-Lung
Cheng Soo-Chen
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
1098-5549
DOI
10.1128/MCB.01213-07
Published
2007-12-00
Epub
2007-00-24
Pages
8027-37
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC2169193
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.01213-07 PMC Full Text View journal details
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